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AAT Bioquest

Why is cross-bridge cycling important in muscle contraction?

Posted October 26, 2023


Answer

Cross-bridge cycling refers to the process by which myosin heads in muscle cells alternately bind to and detach from actin filaments. A cross-bridge is formed when a myosin head attaches to an actin filament. This actin-myosin cross-bridge formation pulls the actin inward, shortening the sarcomere length, which results in a contraction. The energy for the actin-myosin cross-bridge formation is provided by ATP. Immediately after the cross-bridge is formed, the myosin head detaches from the actin filament, breaking the cross-bridge. This repeated attachment and detachment of myosin and actin is known as cross-bridge cycling. It is identified in all muscle types, including skeletal, cardiac, and smooth muscles.

It is important for generating continuous and repetitive muscle contractions and relaxations. 

Additional resources

Myosin cross-bridge kinetics slow at longer muscle lengths during isometric contractions in intact soleus from mice

ATP & ADP

PhosphoWorks™ Fluorimetric ATP Assay Kit