logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
ANOVA Calculator
The one-way analysis of variance (ANOVA) is an extension of independent two-sample t-test is used in studying differences between two or more group means, while the two-way ANOVA evaluates simultaneously the effect of two grouping variables on a response variable. ANOVA uses F-statistic and its corresponding p-value to determine whether data comes from the same population. A significant p-value suggests that some of the group means are different. Being an omnibus test statistic, ANOVA can’t be used to determine which specific groups were statistically significantly different from each other. Hence post hoc tests, such as Tukey multiple pairwise-comparisons and Pairwise t-test are used in evaluating mean difference between specific pairs of groups. The model assumes that each factor is randomly sampled, independent, and belongs to a normally distributed population with unknown but equal variances. The data can be visualized with box plots and bar plots. Assumptions are verified by analyzing model residuals. Simultaneously, multiple comparison tests, such as Shapiro-Wilk, Bartlett, and Flinger are performed to ascertain conclusions on normality, check homogeneity of variances. Finally, alternative analysis to One-way ANOVA includes Welch One-way test that makes no assumptions of equal variances and the non-parametric Kruskal-Wallis rank sum test, which is used when neither assumptions are met.

Data Entry

Feedback
Have a question or a feature request about this tool? Feel free to reach out to us and let us know! We're always looking for ways to improve!

References
This online resource may be cited as follows
MLA
"Quest GraphANOVA Calculator." AAT Bioquest, Inc.21 Sep2025https://www.aatbio.com/tools/anova-analysis-of-variance-one-two-way-calculator.
APA
AAT Bioquest, Inc. (2025September 21). Quest GraphANOVA Calculator. AAT Bioquest. https://www.aatbio.com/tools/anova-analysis-of-variance-one-two-way-calculator.
BibTeXEndNoteRefMan
This online resource has been cited in 7 publications, including
A Paramagnetic Metal-Organic Framework Enhances Mild Magnetic Hyperthermia Therapy by Downregulating Heat Shock Proteins and Promoting Ferroptosis via Aggravation of Two-Way Regulated Redox Dyshomeostasis
Authors: 
Wang, Yi and Chen, Zelong and Li, Jiahui and Wen, Yafei and Li, Jiaxuan and Lv, Yinghua and Pei, Zhichao and Pei, Yuxin
Journal: 
Advanced Science (2023): 2306178
A Blinded Comparative Study of Four Commercially Available LEDs and a Laser Light Curing Device
Authors: 
Comisi, John C and Maucoski, Cristiane and Beller, Jonathan P and Dennis, Kyle S and Price, Richard B
Journal: 
European Journal of Dentistry (2022)
Therapeutic effects of Lucilia sericata larval excretion/secretion products on Leishmania major under in vitro and in vivo conditions
Authors: 
Sherafati, Jila and Dayer, Mohammad Saaid and Ghaffarifar, Fatemeh
Journal: 
Parasites \& Vectors (2022): 1--12
Human identification system using 3D skeleton-based gait features and LSTM model
Authors: 
Rashmi, M and Guddeti, Ram Mohana Reddy
Journal: 
Journal of Visual Communication and Image Representation (2022): 103416