Bradford Protein Assay Calculator

The Bradford Protein Assay is a one-step, nearly instantaneous analytical procedure used to measure the concentration of protein in a solution. It is a colorimetric protein assay based on an absorbance shift of Coomassie Brilliant Blue G-250 from red to blue, the degree to which is proportional to protein concentration. The Bradford Protein Assay has an advantage over direct absorbance measurements, such as using OD280, in that it is less susceptible to interference by various chemical compounds like carbohydrates.

As with most colorimetric or fluorimetric assay, the Bradford Protein Assay requires the generation of a standard curve to calculate the unknown concentration of protein in a solution. The standard curve is created by measuring the absorbances of a known set of protein standards, which are either provided by the manufacturer of the assay or can be easily generated through serial dilution of a stock solution. Include link to serial dilution calculator here. A best fit regression model is applied to the protein standards and their respective response values. This regression model can then be used to calculate the concentration of any unknown protein sample.

This tool will generate a curvi-linear regression model for any given experimental data set with which to determine an unknown protein concentration for the Bradford Protein Assay.