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Image Channel Splitter (RGB)

This tool automatically splits any image/photo into corresponding RBG channels. Background-correction can also be applied by choosing a point on the image to serve as the background color. Intensity of a particular channel is given as the total intensity ÷ total area.

For technical assistance on using this tool, please contact websupport@aatbio.com.

Upload An Image To Begin

(Click image to select)
Position (x, y):x, y
Color Key (R, G, B):r, g, b
Color:

X:
Y:
Red:
Green:
Blue:
Alpha:-
Color:

Intensity (0↧ - 255↥):
Width:
Height:
Size:
File:
Waiting for upload
Feedback
Have a question or a feature request about this tool? Feel free to reach out to us and let us know! We're always looking for ways to improve!
References
This online resource may be cited as follows
MLA
"Quest Image™ Image Channel Splitter (RGB)." AAT Bioquest, Inc., 27 Jul. 2026, https://www.aatbio.com/tools/online-image-channel-splitter-rgb.
APA
AAT Bioquest, Inc. (2026, July 27). Quest Image™ Image Channel Splitter (RGB). AAT Bioquest. https://www.aatbio.com/tools/online-image-channel-splitter-rgb.
BibTeXEndNoteRefMan

  • Click the "Upload Image" button and select a file from your computer or smartphone.
    Valid photo/image formats include: jpg/jpeg, png, gif, bmp and tiff.
    Please note that, in order to ensure consistent performance for all users, image file sizes should be under 5MB and dimensions over 1000 x 1000 pixels will automatically be resized.
    Wait for image to process. The progress bar at the bottom of the tool will indicate when image processing is complete.
    • Additional operations:
  • To determine the RGB value of any point on the image
    Place the crosshairs at the desired point on the image. The crosshairs can be moved either by clicking on the right-hand side preview image directly, by using the sliders provided, or by entering a value into the table below the sliders (0 to 100). The table underneath the sliders will then update with the corresponding RGB value at the indicated point.
  • To perform a background correction
    Place the crosshairs at a point on the image which represents the image's background color (refer to the previous step for instructions on manipulating the crosshairs). Click the "Background Correct" button and confirm operation. Upon successful completion, the final image in the sidebar will contain the original image with full background correction applied.
  • To remove a green screen or set transparency
    Place the crosshairs at a point on the image which represents the image's background color (refer to the previous step for instructions on manipulating the crosshairs). Click the "Transparency" button and confirm operation. Upon successful completion, the final image in the sidebar will contain the original image with full transparency applied.