Validate DNA Sequence

Use this tool to verify that your nucleotide sequence is correctly formatted with proper nucleotide codes. Spaces, tabs, and line breaks will be ignored.

Enter DNA Sequence (ie. AGCT)

DNARNA

Feedback

Have a question or a feature request about this tool? Feel free to reach out to us and let us know! We're always looking for ways to improve!

Email address

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Validate DNA Sequence." AAT Bioquest, Inc., 6 Jan. 2026, https://www.aatbio.com/tools/validate-dna-sequence.
APA	AAT Bioquest, Inc. (2026, January 6). Quest Calculate™ Validate DNA Sequence. AAT Bioquest. https://www.aatbio.com/tools/validate-dna-sequence.
BibTeX	EndNote	RefMan

DNA Molecular Weight
Calculate the molecular weight of a DNA sequence
DNA, RNA Purity
Use absorbance ratio to determine nucleic acid purity
Sense / Anti-Sense
Convert between sense and anti-sense sequences
Sequence Validation
Check that DNA sequence contains all valid letters

Related tools