Ac-DEVD-aminoluciferin
Ordering information
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Additional ordering information
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Physical properties
Molecular weight | 779.79 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 18000 |
Excitation (nm) | 362 |
Emission (nm) | 499 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
Molecular weight 779.79 | Extinction coefficient (cm -1 M -1) 18000 | Excitation (nm) 362 | Emission (nm) 499 |
Ac-DEVD-aminoluciferin is a luminogenic substrate that measures caspase-3 and -7 activities in apoptotic cells. Caspases, a family of aspartate-specific cysteine proteases, are key regulators of apoptosis, orchestrating the initiation and execution of cellular disassembly through the cleavage of specific cellular proteins. Notably, caspases-3 and -7, target the DEVD motif in poly(ADP-ribose) polymerase (PARP), disrupting the repair capabilities of PARP and facilitating DNA fragmentation, a hallmark of apoptosis. When caspase-3/7 enzymes cleave the Ac-DEVD-aminoluciferin substrate, it releases aminoluciferin, which can react with luciferase to produce bioluminescence. The intensity of the bioluminescent signal is directly proportional to the amount of caspase-3/7 activity in the sample, making it a valuable tool for researchers to study and quantify apoptotic processes, screen potential drug candidates, and investigate various cellular pathways related to cell death and survival.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Ac-DEVD-aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 128.24 µL | 641.198 µL | 1.282 mL | 6.412 mL | 12.824 mL |
5 mM | 25.648 µL | 128.24 µL | 256.479 µL | 1.282 mL | 2.565 mL |
10 mM | 12.824 µL | 64.12 µL | 128.24 µL | 641.198 µL | 1.282 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Extinction coefficient (cm -1 M -1) | 18000 |
Excitation (nm) | 362 |
Emission (nm) | 499 |
Product Family
Name | Excitation (nm) | Emission (nm) |
Ac-DEVD-AFC *CAS 201608-14-2* | 376 | 482 |
Ac-DEVD-AMC *CAS 169332-61-0* | 341 | 441 |
Z-DEVD-aminoluciferin | 362 | 499 |