Z-VAD-FMK

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	467.49
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Example protocol

PREPARATION OF STOCK SOLUTIONS

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

Z-VAD-FMK stock solution

Add appropriate amount of DMSO into Z-VAD-FMK to make 2-5 mM Z-VAD-FMK stock solution.
Note Store the unused Z-VAD-FMK stock solution at -20 °C in single use aliquots.

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be used as a guideline and should be optimized according to your needs.

Treat your samples as desired to induce apoptosis.
Add Z-VAD-FMK along with the treatment of your choice.
Note We recommend using 10-100 µM of final concentration ofZ-VAD-FMK to inhibit caspases such as 50 µM for caspase-8.
Note Optimal time and concentration for incubation needs to be determined experimentally.
Stain samples as you desired.
Monitor the fluorescence intensity with a proper instrument.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Z-VAD-FMK to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	213.908 µL	1.07 mL	2.139 mL	10.695 mL	21.391 mL
5 mM	42.782 µL	213.908 µL	427.817 µL	2.139 mL	4.278 mL
10 mM	21.391 µL	106.954 µL	213.908 µL	1.07 mL	2.139 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Product Family

Name	Excitation (nm)	Emission (nm)
FAM-VAD-FMK	493	517
TF4-VAD-FMK	578	602
SRB-VAD-FMK [Sulforhodamine B-VAD-FMK]	559	577

Images

Figure 1. Inhibition of caspase activities in Jurkat cells with pan caspase inhibitor Z-VAD-FMK (Cat.# 13300). Jurkat cells were seeded on the same day at 200,000 cells/90 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with or without 1 µM of staurosporine for 5 hours. The Z-VAD-FMK (50 uM) was added and co-incubated with staurosporine. The caspase-8 activity was detected with Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit (Cat.# 22816). The fluorescence intensity was measured at Ex/Em = 540/620 nm (Cutoff = 610 nm) with Flexstation 3 fluorescence microplate reader (Molecular Devices).

Figure 2. Chemical structure for Z-VAD-FMK.

References

View all 8 references: Citation Explorer

Identification of active caspases using affinity-based probes.
Authors: McStay, Gavin P and Green, Douglas R
Journal: Cold Spring Harbor protocols (2014): 856-60

Induction of apoptosis in human renal cell carcinoma cells by vitamin E succinate in caspase-independent manner.
Authors: Wu, Xiu-Xian and Kakehi, Yoshiyuki and Jin, Xing-Hua and Inui, Masashi and Sugimoto, Mikio
Journal: Urology (2009): 193-9

Metformin induces apoptosis of pancreatic cancer cells.
Authors: Wang, Luo-Wei and Li, Zhao-Shen and Zou, Duo-Wu and Jin, Zhen-Dong and Gao, Jun and Xu, Guo-Ming
Journal: World journal of gastroenterology (2008): 7192-8

Induction of apoptosis in renal tubular cells by histone deacetylase inhibitors, a family of anticancer agents.
Authors: Dong, Guie and Wang, Lysa and Wang, Cong-Yi and Yang, Tianxin and Kumar, M Vijay and Dong, Zheng
Journal: The Journal of pharmacology and experimental therapeutics (2008): 978-84

Apoptosis of gastric cancer cell line MKN45 by photodynamic treatment with photofrin.
Authors: Takahira, Kenichiro and Sano, Munetaka and Arai, Hajime and Hanai, Hiroyuki
Journal: Lasers in medical science (2004): 89-94

Non-caspase-mediated apoptosis contributes to the potent cytotoxicity of the enediyne antibiotic lidamycin toward human tumor cells.
Authors: Wang, Zhen and He, Qiyang and Liang, Yunyan and Wang, Daishu and Li, Yi Yang and Li, Diandong
Journal: Biochemical pharmacology (2003): 1767-75

Androgen induced cell death in SHSY5Y neuroblastoma cells expressing wild-type and spinal bulbar muscular atrophy mutant androgen receptors.
Authors: Grierson, A J and Shaw, C E and Miller, C C
Journal: Biochimica et biophysica acta (2001): 13-20

Critical role for caspases 3 and 8 in neutrophil but not eosinophil apoptosis.
Authors: Daigle, I and Simon, H U
Journal: International archives of allergy and immunology (2001): 147-56