Cell Meter™ Colorimetric MTS Cell Proliferation Kit

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

The Cell Meter™ Colorimetric MTS Cell Proliferation Kit is a quick and reliable method for quantifying viable cells in proliferation and cytotoxicity assays. This technique relies on the reduction of the MTS tetrazolium compound by living cells, which produces a colored formazan product soluble in cell culture media. This reaction is believed to be mediated by NAD(P)H-dependent dehydrogenase enzymes in metabolically active cells. The amount of formazan dye generated by viable cells can be measured by assessing the absorbance at 490-500 nm. The Cell Meter™ Colorimetric MTS Cell Proliferation Kit is useful for evaluating cell proliferation in response to various stimuli such as growth factors, cytokines, mitogens, and nutrients. It is also applicable for analyzing the effects of cytotoxic compounds, including anticancer drugs and other toxic agents and pharmaceutical compounds. A key advantage of this kit is its ease of use, as it can be applied directly to cell culture media, bypassing the intermediate steps required by traditional MTT assays. Furthermore, this high-throughput assay simplifies the process by eliminating the need for washing or solubilization steps and is suitable for use in 96-well microtiter plates.

Platform

Absorbance microplate reader

Absorbance	490 nm
Recommended plate	Clear bottom

Example protocol

AT A GLANCE

Protocol Summary

Prepare the cells in a clear-bottom, 96-well plate (100 µL/well).
Add 20 µL of the MTS Solution to each well.
Incubate at 37°C for 1 - 4 hours.
Monitor absorbance at OD = 460 nm.

CELL PREPARATION

For guidelines on cell sample preparation, please visit:

https://www.aatbio.com/resources/guides/cell-sample-preparation.html

SAMPLE EXPERIMENTAL PROTOCOL

Cell Proliferation and Cytotoxicity Assay

Plate 5,000 to 10,000 cells/well in a tissue culture microplate with a clear bottom.
Add the test compounds to the cells and incubate for a desired period of time (such as 24, 48, or 96 hours) in a 37°C, 5% CO2 incubator. For blank wells (medium without the cells), add the same amount of test compounds. The suggested total volume is 100 µL for a 96-well plate or 50 µL for a 384-well plate.

Note: Each cell line should be evaluated on an individual basis to determine the optimal cell density for proliferation or cytotoxicity induction. For proliferation assays, use fewer cells; for cytotoxicity assays, use more cells to start with.
Add 20 µL/well (96-well plate) or 10 µL/well (384-well plate) of the MTS Solution to each well.
Incubate the plate at 37°C for 1 - 4 hours, protect from light.

Note: The incubation time could be from 30 minutes to overnight depending on the individual cell type and cell concentration used. Optimize the incubation time for each experiment.
Monitor the absorbance increase with an absorbance plate reader at OD = 490 nm.

Cell Counting Assay

Use a clear-bottomed tissue culture microplate to prepare cell culture. The suggested total volume is 100 µL for a 96-well plate or 50 µL for a 384-well plate.

Note: We used serially diluted HeLa and Jurkat cell suspension in a clear bottom 96-well plate for the assay
Add 20 µL/well (96-well plate) or 10 µL/well (384-well plate) of the MTS Solution to each well.
Incubate the plate at 37°C for 1 - 4 hours, protect from light.

Note: The incubation time could be from 30 minutes to overnight depending on the individual cell type and cell concentration used. Optimize the incubation time for each experiment.
Monitor the absorbance increase with an absorbance plate reader at OD =490 nm.

Images

Figure 1. Cell count measured using the Cell Meter™ Colorimetric MTS Cell Quantification Kit. HeLa cells ranging from 781 to 50,000 cells/well/100 µL were added to a clear bottom 96-well plate. After incubation with the MTS solution for 4 hours, the absorbance was measured at 490 nm using a ClarioStar absorbance microplate reader.

References

View all 71 references: Citation Explorer

Assessing Acanthamoeba cytotoxicity: comparison of common cell viability assays.
Authors: Loufouma Mbouaka, Alvie and Lesiak-Markowicz, Iwona and Heredero-Bermejo, Irene and Mazumdar, Rounik and Walochnik, Julia and Martín-Pérez, Tania
Journal: Frontiers in microbiology (2023): 1175469

Evaluation of the chemical, physical, and biological properties of a newly developed bioceramic cement derived from cockle shells: an in vitro study.
Authors: Wannakajeepiboon, Monthip and Sathorn, Chankhrit and Kornsuthisopon, Chatvadee and Santiwong, Busayarat and Wasanapiarnpong, Thanakorn and Linsuwanont, Pairoj
Journal: BMC oral health (2023): 354

Effect of exposure conditions on chemical properties of materials for surgical endodontic procedures.
Authors: Koutroulis, Andreas and Valen, Håkon and Ørstavik, Dag and Kapralos, Vasileios and Camilleri, Josette and Sunde, Pia Titterud
Journal: European journal of oral sciences (2023): e12943

Methotrexate loaded in alginate beads for controlled drug release against breast cancer.
Authors: Taran, Zahra and Yektaniroumand Digehsaraei, Sepideh and Salouti, Mojtaba and Amini, Bahram and Mahmazi, Sanaz and Kalantari, Mohsen
Journal: Gene (2023): 146941

Enhanced chemotherapeutic efficacy of docetaxel in human lung cancer cell line via GLUT1 inhibitor.
Authors: Bahremani, Mona and Rashtchizadeh, Nadereh and Sabzichi, Mehdi and Vatankhah, Amir Mansour and Danaiyan, Sepideh and Poursistany, Haniyeh and Mohammadian, Jamal and Ghorbanihaghjo, Amir
Journal: Journal of biochemical and molecular toxicology (2023): e23348

Evaluation of the Cytocompatibility of Fluoride Varnish and Its Effect on Human Gingival Fibroblasts (hGFs): An In Vitro Study.
Authors: Seshadri, V Ranjith Akshay and Varghese, Nikitha S and Gurunathan, Deepa
Journal: Cureus (2023): e41735

Use of Saponinosomes from Ziziphus spina-christi as Anticancer Drug Carriers.
Authors: Nazemoroaya, Zahra and Sarafbidabad, Mohsen and Mahdieh, Athar and Zeini, Darya and Nyström, Bo
Journal: ACS omega (2022): 28421-28433

Inhibition of Methamphetamine-Induced Cytotoxicity in the U87-Cell Line by Atorvastatin-Conjugated Carbon Nanotubes.
Authors: Nikeafshar, Sara and Khazaei, Ardeshir and Tahvilian, Reza
Journal: Applied biochemistry and biotechnology (2022): 479-503

Time Depletion Effects on the Volatile Compounds from the Distillation Extracts of Prunella vulgaris and the Dynamics of their Extraction.
Authors: Mak, William Chi Keung
Journal: Current drug research reviews (2022): 148-156

New inhibitors of cathepsin V impair tumor cell proliferation and elastin degradation and increase immune cell cytotoxicity.
Authors: Mitrović, Ana and Senjor, Emanuela and Jukić, Marko and Bolčina, Lara and Prunk, Mateja and Proj, Matic and Nanut, Milica Perišić and Gobec, Stanislav and Kos, Janko
Journal: Computational and structural biotechnology journal (2022): 4667-4687

Documents

Safety data sheet
Product protocol
Certificate of analysis

Location: Home / Products / Cell Meter™ Colorimetric MTS Cell Proliferation Kit

About Privacy Terms of Use Terms of Sales Distributors