Nuclear Green™ Fixable DCS1 *5 mM DMSO Solution*
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Solvent | DMSO |
Excitation (nm) | 510 |
Emission (nm) | 532 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Overview | SDSProtocol |
Excitation (nm) 510 | Emission (nm) 532 |
Platform
Fluorescence microscope
Excitation | FITC filter set |
Emission | FITC filter set |
Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Ex/Em = 510/532 nm (bound to DNA)
SAMPLE EXPERIMENTAL PROTOCOL
Caution: The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types, and factors may influence staining. Residual detergent on glassware may also affect the staining of many organisms and cause brightly stained material to appear in solutions with or without cells present.
Add 2 to 10 µM of Nuclear Green™ DCS1 to fixed, dead, or apoptotic cells (whether in suspension or adherent) and incubate for 15 to 60 minutes.
Note: In order to determine the optimal concentration that yields the desired result, it is advisable to test a wide range of dye concentrations in initial experiments.
Optional: Wash the cells twice with Hanks and 20 mM HEPES buffer (HBSS) or a buffer of your choice. Then fill the wells with fresh HBSS or growth medium.
Observe the cells using a fluorescence microscope, fluorescence microplate reader, or flow cytometer equipped with the desired filter set.
Optional: Fix cells with 4% formaldehyde for 20 minutes at room temperature. Wash cells twice to get rid of any fixation solution
Images
References
Authors: Wang, Xiaoyan and Qiao, Yinuo and Zhang, Jinyang and Song, Yuzhu and Han, Qinqin
Journal: Analytical and bioanalytical chemistry (2024): 299-311
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Authors: Fujii, Koji and Mita, Yusuke and Watahiki, Haruhiko and Fukagawa, Takashi and Kitayama, Tetsushi and Mizuno, Natsuko and Nakahara, Hiroaki and Sekiguchi, Kazumasa
Journal: Legal medicine (Tokyo, Japan) (2023): 102206
Authors: Chen, Max Y and Heinrich, Laurin and Zafar, Faria and Sedov, Kamilla and Schüle, Birgitt
Journal: Bio-protocol (2023): e4689
Authors: Nouroloyouni, Ahmad and Moghaddam, Negar and Nuroloyuni, Sarah and Milani, Amin Salem and Yavari, Hamid Reza and Majidi, Ali Reza
Journal: Journal of dentistry (Shiraz, Iran) (2023): 429-437