Nuclear Blue™ LCS1

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	822.69
Solvent	DMSO

Spectral properties

Excitation (nm)	353
Emission (nm)	456

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Overview SDS Protocol

Molecular weight

822.69

Excitation (nm)

353

Emission (nm)

456

Our Nuclear Blue™ LCS1 is a fluorogenic, DNA-selective, and cell-permeant dye for analyzing DNA content in live cells. The Nuclear Blue™ LCS1 has its blue fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate, and flow cytometry applications. This DNA-binding dye might be used for the multicolor analysis of live cells with the DAPI filter sets. For example, Nuclear Blue™LCS1 can be used with GFP cell lines.

Platform

Fluorescence microscope

Excitation	DAPI Filter
Emission	DAPI Filter
Recommended plate	Black wall/clear bottom

Example protocol

AT A GLANCE

Spectral Properties

Ex/Em = 353/456 nm (bound to DNA)

SAMPLE EXPERIMENTAL PROTOCOL

Caution: The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types, and factors may influence staining. Residual detergent on glassware may also affect the staining of many organisms and cause brightly stained material to appear in solutions with or without cells present.

Add Nuclear Blue™ LCS1 (2 to10 µM) directly into the live cell culture medium (either suspension or adherent) and incubate the cells for 15 to 60 minutes.

Note: In initial experiments, it is advisable to test a wide range of dye concentrations in order to determine the optimal concentration that yields the desired result.
Wash the cells twice with Hanks and 20 mM HEPES buffer (HBSS) or a buffer of your choice. Then fill the wells with fresh HBSS or growth medium.
Observe the cells using a fluorescence microscope, fluorescence microplate reader, or flow cytometer equipped with the desired filter set.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nuclear Blue™ LCS1 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	121.552 µL	607.762 µL	1.216 mL	6.078 mL	12.155 mL
5 mM	24.31 µL	121.552 µL	243.105 µL	1.216 mL	2.431 mL
10 mM	12.155 µL	60.776 µL	121.552 µL	607.762 µL	1.216 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	353
Emission (nm)	456

Product Family

Name	Excitation (nm)	Emission (nm)
Nuclear Blue™ DCS1 5 mM DMSO Solution	348	469
Nuclear Blue™ DCS2 Equivalent to SYTOX™ Blue Dead Cell Stain	445	470
Nuclear Green™ LCS1 5 mM DMSO Solution	503	527
Nuclear Orange™ LCS1 5 mM DMSO Solution	514	556
Nuclear Red™ LCS1 5 mM DMSO Solution	622	645
Nuclear Violet™ LCS1 5 mM DMSO Solution	401	460

Images

Figure 1. Fluorescence image of live HeLa cells stained with Nuclear Blue™ LCS1 (Cat. 17559) and visualized using a fluorescence microscope equipped with a DAPI filter set.

References

View all 50 references: Citation Explorer

Breaking a Dogma: High-Throughput Live-Cell Imaging in Real-Time with Hoechst 33342.
Authors: Fuchs, Heiko and Jahn, Kirsten and Hu, Xiaonan and Meister, Roland and Binter, Maximilian and Framme, Carsten
Journal: Advanced healthcare materials (2023): e2300230

An annotated high-content fluorescence microscopy dataset with Hoechst 33342-stained nuclei and manually labelled outlines.
Authors: Arvidsson, Malou and Rashed, Salma Kazemi and Aits, Sonja
Journal: Data in brief (2023): 108769

Hoechst 33342 as a marker for imaging neurites of Dorsal Root Ganglion in vitro.
Authors: Merolli, Antonio and Bektas, Cemile
Journal: Journal of anatomy (2022): 998-1001

The pH and mercury ion stimuli-responsive supramolecular assemblies of cucurbit[7]uril and Hoechst 33342.
Authors: Wang, Qin and Lü, Li-Bing and Tao, Zhu and Sun, Tao and Tang, Qing and Huang, Ying
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 119656

Complexities of a protonatable substrate in measurements of Hoechst 33342 transport by multidrug transporter LmrP.
Authors: Swain, Brendan M and Guo, Dawei and Singh, Himansha and Rawlins, Philip B and McAlister, Mark and van Veen, Hendrik W
Journal: Scientific reports (2020): 20026

Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining.
Authors: Eddaoudi, Ayad and Canning, Stephanie Louise and Kato, Itaru
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 49-57

Photoselection of Luminescent Molecules in Anisotropic Media in the Case of Two-Photon Excitation. Part II. Experimental Studies of Hoechst 33342 in Stretched Poly(vinyl alcohol) Films.
Authors: Kawski, A and Gryczyński, Z and Gryczyński, I and Lakowicz, J R and Piszczek, G
Journal: Zeitschrift fur Naturforschung. A, Journal of physical sciences (2016): 1037-1041

Labeling Adipose-Derived Stem Cells with Hoechst 33342: Usability and Effects on Differentiation Potential and DNA Damage.
Authors: Schendzielorz, P and Froelich, K and Rak, K and Gehrke, T and Scherzad, A and Hagen, R and Radeloff, A
Journal: Stem cells international (2016): 6549347

Cell Cycle Analysis of CML Stem Cells Using Hoechst 33342 and Propidium Iodide.
Authors: DeSouza, Ngoc and Zhou, Megan and Shan, Yi
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 47-57

Analyzing Cell Death by Nuclear Staining with Hoechst 33342.
Authors: Crowley, Lisa C and Marfell, Brooke J and Waterhouse, Nigel J
Journal: Cold Spring Harbor protocols (2016)