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AAT Bioquest

Application Notes

 


Flow cytometry is a technology used to rapidly analyze cells or particles in a buffered salt-based solution. Flow cytometry uses one, or multiple, lasers to determine the visible light scatter given off by each cell. This light scatter is measured in two specific directions, forward and sideways,

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Posted May 2, 2024

Cell lysis, also known as cell disruption, is a common laboratory technique aimed at extracting target nucleic acids or proteins inside the cell. In cell lysis or cellular disruption is a method in which the cell membrane is broken down or destroyed. In this process, subcellular components like DNA,

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Posted April 30, 2024

Chimeric antigen receptor (CAR) T-cell therapy is an innovative approach to the treatment of cancer, immunodeficiencies, and various other hematologic malignancies. The CAR is a lab-built technology that uses three main domains to bind to certain proteins and/or cancer cells; an extracellular

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Posted April 29, 2024

Solubility is the degree in which a substance (termed “solute”) dissolves into a solvent, thereby forming a solution. Solubility is often measured in grams of solute per 100 mL of solvent, at a particular temperature. Solutes and solvents can both exist in various forms, like solids, liquids, gas,

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Posted April 22, 2024

DNA extraction is the method by which nuclear DNA is isolated from all other parts of the cell. DNA extraction can be physical or chemical in nature, and can be performed on cultured cells, animal tissues, plants, and bacteria. Though some steps can be adjusted to better suit the desired experimental

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Posted April 4, 2024