Calculator
How does the streptavidin-biotin (LSAB) method work?
Posted May 8, 2024
Answer
- The tissue is exposed to the primary antibody, which attaches specifically to the antigen of interest.
- A biotin-labeled secondary antibody is introduced, binding to the primary antibody already attached to the antigen.
- An enzyme-labeled streptavidin complex is applied, where streptavidin directly binds to enzyme molecules. This complex then binds to the biotin-labeled secondary antibody.
- Lastly, a chromogenic substrate is added to reveal the peroxidase enzyme, allowing visualization of the antigen site.
Additional resources
Immunohistochemistry in investigative and toxicologic pathology