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What are the advantages of indirect detection methods in immunohistochemistry (IHC)?
Posted May 2, 2024
Answer
- This process provides a stronger signal because it uses multiple secondary antibodies, which bind to each primary antibody.
- The type of label or detection techniques used can be changed easily for new assays by changing secondary antibodies.
- Researchers can choose from a wider variety of labels.
- Making all primary antibodies in the same species can save labeling time and total cost.
- The use of biotinylated secondary antibodies amplifies sensitivity and signal even further while also allowing for greater flexibility.
Additional resources
Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry