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What are the disadvantages of indirect detection methods in immunohistochemistry (IHC)?

Posted May 2, 2024


Antibodies and Proteomics
Bioconjugation
Antibody and Protein Labeling
Immunohistochemistry (IHC)
Answer
  • The binding between the secondary antibody and other proteins present on the blot can give rise to more non-specific signals. 
  • Additional incubation, blocking, and wash steps add time to the process.  
Additional resources

Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry

Immunohistochemistry (IHC)

Stayright™ Purple HRP Staining Kit

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Location: Home / Frequently Asked Questions (FAQ) / What are the disadvantages of indirect detection methods in immunohistochemistry (IHC)?
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