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Which enzyme is most often used in blotting procedures?

Posted May 2, 2024


Antibodies and Proteomics
Enzymes
Horseradish Peroxidase (HRP) and Poly-HRP
Immunohistochemistry (IHC)
Secondary Antibodies
Answer

Horse-radish peroxidase (HRP) or phosphatase-labeled secondary antibodies are the most popular enzymes used in blotting procedures because they generate colorful products when combined with the substrate during detection. 

Additional resources

Overview of Western Blotting

Western Blotting Assays

Horseradish Peroxidase (HRP) and Poly-HRP

MegaWox™ polyHRP-Goat Anti-Mouse IgG Conjugate

Related questions
Why is horseradish peroxidase the enzyme that is used most often in blotting procedures?
How do I know if an antibody is primary or secondary?
Why is an HRP conjugated secondary antibody used in western blotting?
Are there different IgG antibodies?
Can I use an HRP-conjugated primary antibody for western blot (with no secondary)?
Location: Home / Frequently Asked Questions (FAQ) / Which enzyme is most often used in blotting procedures?
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