OG488 BAPTA-1, hexapotassium salt [equivalent to Oregon Green® 488 BAPTA-1, hexapotassium salt] Cell impermeant

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Dissociation constant (K_d, nM)	170
Molecular weight	1114.28
Solvent	Water

Spectral properties

Excitation (nm)	493
Emission (nm)	522

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

1114.28

Dissociation constant (K_d, nM)

170

Excitation (nm)

493

Emission (nm)

522

OG488 BAPTA -1 is the same molecule of Oregon Green 488 BAPTA-1. It is a visible light-excitable calcium indicator that is often used with FITC filter set. OG 488 BAPTA-1 has excitation and emission at 494 and 523 nm respectively. Its fluorescence intensity increases by ~14 fold upon binding calcium ion. This water-soluble, salt form is useful for intracellular loading by microinjection or infusion from patch pipette. The cell-permeant AM ester form of the indicator is also available (#20507).

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of OG488 BAPTA-1, hexapotassium salt [equivalent to Oregon Green® 488 BAPTA-1, hexapotassium salt] *Cell impermeant* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	89.744 µL	448.72 µL	897.44 µL	4.487 mL	8.974 mL
5 mM	17.949 µL	89.744 µL	179.488 µL	897.44 µL	1.795 mL
10 mM	8.974 µL	44.872 µL	89.744 µL	448.72 µL	897.44 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	493
Emission (nm)	522

Images

Figure 1. Chemical structure for OG488 BAPTA-1, hexapotassium salt [equivalent to Oregon Green® 488 BAPTA-1, hexapotassium salt] *Cell impermeant*

References

View all 38 references: Citation Explorer

Norepinephrine-induced calcium signaling in astrocytes in the respiratory network of the ventrolateral medulla
Authors: Schnell C, Negm M, Driehaus J, Scheller A, Hulsmann S.
Journal: Respir Physiol Neurobiol (2016): 18

Optical electrocorticogram (OECoG) using wide-field calcium imaging reveals the divergence of neuronal and glial activity during acute rodent seizures
Authors: Daniel AG, Laffont P, Zhao M, Ma H, Schwartz TH.
Journal: Epilepsy Behav (2015): 61

Two-Photon Processor and SeNeCA: a freely available software package to process data from two-photon calcium imaging at speeds down to several milliseconds per frame
Authors: Tomek J, Novak O, Syka J.
Journal: J Neurophysiol (2013): 243

Calcium buffering and clearance in spider mechanosensory neurons
Authors: Schmitz J, Hoger U, Torkkeli PH, French AS.
Journal: J Comp Physiol A Neuroethol Sens Neural Behav Physiol (2012): 477

Post hoc immunostaining of GABAergic neuronal subtypes following in vivo two-photon calcium imaging in mouse neocortex
Authors: Langer D, Helmchen F.
Journal: Pflugers Arch (2012): 339

Astrocyte calcium signaling transforms cholinergic modulation to cortical plasticity in vivo
Authors: Takata N, Mishima T, Hisatsune C, Nagai T, Ebisui E, Mikoshiba K, Hirase H.
Journal: J Neurosci (2011): 18155

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377

Multifocal animated imaging of changes in cellular oxygen and calcium concentrations and membrane potential within the intact adult mouse carotid body ex vivo
Authors: Wotzlaw C, Bernardini A, Berchner-Pfannschmidt U, Papkovsky D, Acker H, F and rey J., undefined
Journal: Am J Physiol Cell Physiol (2011): C266

Somatic depolarization enhances GABA release in cerebellar interneurons via a calcium/protein kinase C pathway
Authors: Bouhours B, Trigo FF, Marty A.
Journal: J Neurosci (2011): 5804

Voltage-gated calcium channels are involved in the regulation of calcium oscillations in vascular smooth muscle cells from isolated porcine retinal arterioles
Authors: Misfeldt MW, Aalkjaer C, Simonsen U, Bek T.
Journal: Exp Eye Res (2010): 69