OG488 BAPTA-1, hexapotassium salt [equivalent to Oregon Green® 488 BAPTA-1, hexapotassium salt] *Cell impermeant*

OG488 BAPTA-1, hexapotassium salt

equivalent to Oregon Green® 488 BAPTA-1, hexapotassium salt; Cell impermeant

OG488 BAPTA -1 is the same molecule of Oregon Green 488 BAPTA-1. It is a visible light-excitable calcium indicator that is often used with FITC filter set. OG 488 BAPTA-1 has excitation and emission at 494 and 523 nm respectively. Its fluorescence intensity increases by ~14 fold upon binding calcium ion. This water-soluble, salt form is useful for intracellular loading by microinjection or infusion from patch pipette. The cell-permeant AM ester form of the indicator is also available (#20507).

Protocol

SDS

Catalog	Size	Price	Quantity
20506	500 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of OG488 BAPTA-1, hexapotassium salt [equivalent to Oregon Green® 488 BAPTA-1, hexapotassium salt] *Cell impermeant* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	89.744 µL	448.72 µL	897.44 µL	4.487 mL	8.974 mL
5 mM	17.949 µL	89.744 µL	179.488 µL	897.44 µL	1.795 mL
10 mM	8.974 µL	44.872 µL	89.744 µL	448.72 µL	897.44 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1114.28 g/mol	=	mL	x	mM	=	mmol

References

View all 38 references: Citation Explorer

Norepinephrine-induced calcium signaling in astrocytes in the respiratory network of the ventrolateral medulla

Authors:

Schnell C, Negm M, Driehaus J, Scheller A, Hulsmann S.

Journal:

Respir Physiol Neurobiol (2016): 18

Optical electrocorticogram (OECoG) using wide-field calcium imaging reveals the divergence of neuronal and glial activity during acute rodent seizures

Authors:

Daniel AG, Laffont P, Zhao M, Ma H, Schwartz TH.

Journal:

Epilepsy Behav (2015): 61

Two-Photon Processor and SeNeCA: a freely available software package to process data from two-photon calcium imaging at speeds down to several milliseconds per frame

Authors:

Tomek J, Novak O, Syka J.

Journal:

J Neurophysiol (2013): 243

Calcium buffering and clearance in spider mechanosensory neurons

Authors:

Schmitz J, Hoger U, Torkkeli PH, French AS.

Journal:

J Comp Physiol A Neuroethol Sens Neural Behav Physiol (2012): 477

Post hoc immunostaining of GABAergic neuronal subtypes following in vivo two-photon calcium imaging in mouse neocortex

Authors:

Langer D, Helmchen F.

Journal:

Pflugers Arch (2012): 339

Physical properties

Dissociation constant (K_d, nM)	170
Molecular weight	1114.28
Solvent	Water

Spectral properties

Excitation (nm)	493
Emission (nm)	522

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Calcium Detection Probes & Assay Kits

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Page updated on November 1, 2025