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AF Dye-CD Antibody Conjugates
AF Dye-CD antibody conjugates are primary antibodies pre-labeled with our XFD dye series. These conjugates are designed for direct immunofluorescence detection of cluster of differentiation (CD) markers on cell surfaces. XFD dyes are spectrally equivalent to Alexa Fluor® dyes, providing researchers with high-quality, ready-to-use reagents for flow cytometry, immunofluorescence microscopy, and immunophenotyping applications. These conjugates eliminate the need for secondary antibody staining, simplifying multicolor panel design and reducing experimental time.
| Fluorophore | Ex/Em (nm) | Laser Line | Best For | Common Filters |
|---|---|---|---|---|
| XFD488 | 493/517 | 488 nm (Blue) | High brightness, standard green channel | FITC |
| XFD532 | 532/553 | 532 nm (Green) | Yellow-green emission, multiplex panels | Cy3 |
| XFD555 | 555/580 | 532/561 nm | Orange emission, excellent photostability | TRITC/Cy3 |
| XFD594 | 590/617 | 561/594 nm | Red emission, low background autofluorescence | Texas Red |
| XFD647 | 650/668 | 633/640 nm (Red) | Far-red emission, minimal spectral overlap | Cy5/APC |
| XFD700 | 696/719 | 633/640 nm (Red) | Near-IR emission, extended multiplex panels | Cy5.5 |
| XFD750 | 749/775 | 633/640 nm (Red) | Near-IR, deep tissue imaging applications | Cy7 |
AF Dye-conjugated CD antibodies are directly labeled primary antibodies targeting cluster of differentiation antigens expressed on immune cells and other cell types. These conjugates utilize AAT Bioquest's XFD dye series, which are bright, photostable fluorescent dyes that are spectrally equivalent to Alexa Fluor® dyes, offering researchers reliable performance for immunophenotyping, cell sorting, and multicolor flow cytometry applications.
- Direct detection format — No secondary antibody required, reducing protocol steps and potential background
- Validated for flow cytometry — Optimized dye-to-protein ratios ensure consistent, reproducible results
- Broad spectral coverage — Available in multiple fluorophore options (XFD488, XFD555, XFD594, XFD647, XFD700, XFD750) for flexible panel design
- Spectrally matched to Alexa Fluor® dyes — Compatible with existing instrument configurations and filter sets
- High photostability — Excellent resistance to photobleaching for extended imaging sessions
- Low background — Minimal non-specific binding for improved signal-to-noise ratios
CD markers serve as critical identifiers for characterizing immune cell populations. The table below summarizes common CD targets and their primary applications in immunophenotyping research.
| CD Marker | Synonyms | Primary Cell Types | Common Applications |
|---|---|---|---|
| CD3 | T3 | T lymphocytes | Pan-T cell marker, T cell activation studies |
| CD4 | T4, Leu-3 | Helper T cells, monocytes | T helper cell identification, HIV research, immunocompetence assessment |
| CD8 | T8, Leu-2 | Cytotoxic T lymphocytes, NK cells | CTL identification, immune monitoring, tumor-infiltrating lymphocyte analysis |
| CD14 | LPS receptor, Monocyte marker | Monocytes, macrophages | Monocyte gating, inflammation studies, bacterial response assessment |
| CD19 | B4 | B lymphocytes | Pan-B cell marker, lymphoma typing, B cell lymphoma classification |
| CD20 | B1, Bp35 | B lymphocytes | B cell development, rituximab therapy monitoring, B cell malignancy detection |
| CD25 | IL-2Ralpha | Activated T cells, regulatory T cells (Tregs) | Regulatory T cell identification, T cell activation marker, immunosuppression studies |
| CD34 | Mucosialin | Hematopoietic stem and progenitor cells | Stem cell enumeration, transplant monitoring, hematopoietic assessment |
| CD45 | LCA (Leukocyte Common Antigen) | All leukocytes | Pan-leukocyte gating, lymphoma diagnosis, immune cell enumeration |
| CD56 | NCAM (Neural Cell Adhesion Molecule) | Natural killer (NK) cells, NKT cells | NK cell identification, immunotherapy monitoring, cancer immunology studies |
The table below lists all available AF Dye-CD antibody conjugates organized by CD target. AF Dye-CD antibody conjugates utilize AAT Bioquest's XFD dyes, which are chemically similar and spectrally equivalent to Alexa Fluor® dyes (Alexa Fluor® is a trademark of ThermoFisher Scientific). Click any clone name to view the product page.
For researchers seeking superior fluorescence performance, AAT Bioquest's iFluor® dye series offers enhanced brightness and photostability compared to traditional fluorophores. iFluor® conjugates are available across the full visible spectrum and represent next-generation alternatives to Alexa Fluor® and other conventional dyes.
- Superior brightness — Up to 2× brighter than comparable Alexa Fluor® dyes in many applications
- Enhanced photostability — Extended imaging without significant signal loss
- Excellent water solubility — Minimal aggregation and non-specific binding
- Optimized for biological applications — Designed specifically for antibody conjugation and cellular imaging
This document (01.0281.251203r1) was last updated on Thu Nov 12 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.