AAT Bioquest offers a specialized selection of colorimetric assays optimized for NanoDrop and micro-volume spectrophotometers. These rapid, convenient assays enable researchers to quantify biotin content, protein aldehyde modifications, and pH levels using minimal sample volumes (1-2 µL). Each product in this category is specifically formulated to provide accurate, reproducible results with micro-volume spectrophotometers, eliminating the need for traditional cuvette-based measurements or plate readers.
Application
Detection Principle
Best For
Product
Biotin Quantitation
Colorimetric (Absorbance)
Determining biotin:protein ratio on conjugates
Amplite® Biotin Kit
Aldehyde Quantitation
Colorimetric (Absorbance)
Quantifying aldehyde modifications on proteins
Amplite® Aldehyde Kit
pH Measurement
Colorimetric (Absorbance)
Micro-volume pH analysis (pH 4.5-8.5)
Spexyte™ Micro pH Probe
Amplite® Quantitation Kits
NanoDrop-compatible Amplite® colorimetric quantitation kits enable rapid, accurate measurement of biotin and aldehyde content on proteins and other macromolecules. These kits utilize proprietary chromogenic reagents that produce stable, measurable absorbance signals. The biotin quantitation kit enables determination of biotin:protein ratios critical for optimizing biotin-streptavidin applications, while the aldehyde content kit supports site-selective protein modification workflows.
NanoDrop-optimized: Specifically formulated for accurate readings on micro-volume spectrophotometers
Minimal sample requirement: Only 1-2 µL of sample needed per measurement
Rapid protocols: Results in minutes without lengthy incubation steps
No plate reader required: Eliminates the need for 96-well plates and microplate readers
Quantitative results: Provides accurate biotin or aldehyde quantitation for conjugate characterization
The Spexyte™ Micro pH Probe is a colorimetric pH indicator specifically optimized for micro-volume spectrophotometers like NanoDrop. This probe enables rapid, accurate pH measurements of biological samples in the physiologically relevant range of pH 4.5 to 8.5 using just 1-2 µL of sample. The probe generates a stable, reproducible absorbance signal that correlates directly with sample pH, making it ideal for quality control of buffers, cell culture media, and other biological preparations where sample volume is limited.
Micro-volume compatible: Measures pH using only 1-2 µL of sample
Physiological pH range: Optimized for biological samples (pH 4.5-8.5)
No electrode required: Eliminates calibration and maintenance of traditional pH meters
This document (01.0251.251203r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.