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Fluorimetric Assays (Qubit™ and Cytocite™)
AAT Bioquest's Fluorimetric Assays for Qubit™ and CytoCite™ fluorometers provide researchers with convenient, ready-to-use quantitation solutions for nucleic acids, proteins, ions, and other analytes. The Portelite™ kit series is specifically optimized for benchtop fluorometers, delivering accurate results with minimal sample volume. Tests are performed in single-tube format, often with pre-calibrated standards, enabling fast sample quantification for experimental validation and quality-control.
Product Group | Example Analyte |
|---|---|
Nucleic Acid Quantitation Kits | dsDNA, ssDNA, total RNA |
Protein Quantitation Kits | Total protein |
Ion Detection Kits | Li⁺, Na⁺ |
Specialty Detection Kits | Biotin, Endotoxin |
Nucleic Acid Quantitation Kits
Portelite™ nucleic acid quantitation kits are specifically optimized for fluorometers including CytoCite™ and Qubit™ instruments. These assays use highly selective fluorescent dyes that bind specifically to their target nucleic acid, providing accurate quantitation even in the presence of common contaminants like proteins, salts, and free nucleotides.
Fig. 1
DNA standard curve generated using Portelite™ Fluorimetric DNA Quantitation Kit with Broad Dynamic Range. Fluorescence intensity was quantified using green fluorescence channel, regression model was calculated using linear fit.
Key Features
- Fluorometer-optimized – Specifically formulated for CytoCite™ and Qubit™ instruments
- High selectivity – Minimal interference from proteins, salts, and free nucleotides
- Ready-to-use reagents – Pre-formulated buffers and standards for fast setup
- Broad and high-sensitivity options – Choose based on sample concentration range
- Compatible with NGS workflows – Accurate quantitation for library prep and sequencing
Protein Quantitation Kit
The Portelite™ Fluorimetric Protein Quantitation Kit provides a rapid, sensitive method for measuring total protein concentration using benchtop fluorometers. The assay utilizes a fluorescent dye that selectively binds to proteins, enabling accurate quantitation without interference from common buffer components.
Key Features
- Broad protein compatibility – Works with diverse protein types including antibodies
- Low sample requirement – Quantify proteins with minimal sample volumes
- Fast protocol – Results in minutes with simple mix-and-read format
- Resistant to common contaminants – Tolerates detergents, reducing agents, and salts
Ion Detection Kits
Portelite™ ion quantification kits enable rapid, sensitive detection of lithium and sodium ions using benchtop fluorometers. These kits utilize proprietary ion-selective indicator dyes (Lithiumighty™ 520 and SoNa™ 520) that exhibit significant fluorescence enhancement upon ion binding.
Key Features
- Proprietary indicator dyes – Lithiumighty™ 520 and SoNa™ 520 for high selectivity
- Clinical applications – Suitable for therapeutic drug monitoring (lithium) and electrolyte analysis
- Instrument compatibility – Optimized for CytoCite™ and Qubit™ fluorometers
- Minimal sample preparation – Simple dilute-and-read protocol
Specialty Detection Kits
Portelite™ specialty kits extend fluorometry to biotin conjugate quantification and endotoxin detection. These assays provide convenient alternatives to traditional methods, enabling rapid quality control checks in conjugation workflows and contamination screening.
Key Features
- Biotin quantification – Measure biotinylation efficiency of conjugates directly
- Endotoxin detection – LAL-free fluorimetric approach for rapid contamination screening
- Instrument compatibility – Optimized for CytoCite™ and Qubit™ fluorometers
- Streamlined QC – Rapid results for production and research workflows
This document (01.0249.251203r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.
Fluorimetric Assays (Qubit™ and Cytocite™)