AAT Bioquest offers fluorogenic substrates for detecting β-glucuronidase (EC 3.2.1.31) enzyme activity in both reporter gene systems and endogenous enzymatic contexts. β-Glucuronidase catalyzes the hydrolysis of β-D-glucuronides, releasing fluorescent reporter molecules that enable sensitive detection in cell extracts, purified enzyme preparations, and reporter gene assays. These substrates are widely used in GUS reporter gene studies in plant and bacterial systems and are also applicable for measuring endogenous β-glucuronidase activity in mammalian cell and tissue extracts.

These fluorogenic substrates are non-fluorescent until cleaved by β-glucuronidase, releasing highly fluorescent reporter molecules. The enzymatic hydrolysis of the glucuronide bond liberates either fluorescein (green fluorescence) or 4-methylumbelliferone (blue fluorescence), enabling quantitative measurement of GUS activity. Both substrates are compatible with standard fluorescence microplate readers and offer excellent signal-to-background ratios for sensitive enzyme detection.