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Histone Deacetylases (HDAC)
Histone deacetylases (HDACs) are epigenetic regulators that remove acetyl groups from lysine residues on histones and other proteins, influencing chromatin structure and gene regulation. Our Amplite® platform provides sensitive, homogeneous detection of HDAC activity for epigenetics research, drug discovery, and inhibitor screening applications.
Amplite® HDAC Activity Assay Kit
The Amplite® Fluorimetric HDAC Activity Assay Kit provides a convenient, homogeneous method for detecting histone deacetylase activity in biological samples. The assay utilizes a proprietary HDAC Green™ substrate that becomes highly fluorescent upon deacetylation by HDAC enzymes. This single-step, no-wash format is ideal for high-throughput screening of HDAC inhibitors and measuring enzyme activity in nuclear extracts.
Key Features
- Homogeneous, no-wash format — Simple mix-and-read protocol ideal for HTS applications
- Non-peptide substrate — Improved stability compared to traditional peptide-based substrates
- Green fluorescence output — Compatible with standard fluorescein filter sets (Ex/Em = 493/520 nm)
- Superior sensitivity — Outperforms competitor assays for detecting HDAC activity in nuclear extracts
- Broad HDAC detection — Detects activity from Class I, II, and IV HDAC enzymes
- Validated for inhibitor screening — Suitable for IC₅₀ determination with inhibitors like Trichostatin A and sodium butyrate
Fig. 1
HDAC activity in HeLa nuclear extract measured with Amplite™ Fluorimetric HDAC Activity Assay Kit (in blue, Catalog Number 13601) was compared with Vendor X (in red) and Vendor Y (in green), both of which use Ac-RGK(Ac)-R110 peptide substrate. The signal/background ratio of the HDAC activity measured with Amplite™ Fluorimetric HDAC Activity Assay Kit is more than10 times higher than those of Vendors X and Y.
This document (01.0270.251203r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.
Histone Deacetylases (HDAC)