Histone Deacetylases (HDAC)

Histone deacetylases (HDACs) are epigenetic regulators that remove acetyl groups from lysine residues on histones and other proteins, influencing chromatin structure and gene regulation. Our Amplite® platform provides sensitive, homogeneous detection of HDAC activity for epigenetics research, drug discovery, and inhibitor screening applications.

Amplite® HDAC Activity Assay Kit

The Amplite® Fluorimetric HDAC Activity Assay Kit provides a convenient, homogeneous method for detecting histone deacetylase activity in biological samples. The assay utilizes a proprietary HDAC Green™ substrate that becomes highly fluorescent upon deacetylation by HDAC enzymes. This single-step, no-wash format is ideal for high-throughput screening of HDAC inhibitors and measuring enzyme activity in nuclear extracts.

Key Features

Homogeneous, no-wash format — Simple mix-and-read protocol ideal for HTS applications
Non-peptide substrate — Improved stability compared to traditional peptide-based substrates
Green fluorescence output — Compatible with standard fluorescein filter sets (Ex/Em = 493/520 nm)
Superior sensitivity — Outperforms competitor assays for detecting HDAC activity in nuclear extracts
Broad HDAC detection — Detects activity from Class I, II, and IV HDAC enzymes
Validated for inhibitor screening — Suitable for IC₅₀ determination with inhibitors like Trichostatin A and sodium butyrate

Fig. 1

HDAC activity in HeLa nuclear extract measured with Amplite™ Fluorimetric HDAC Activity Assay Kit (in blue, Catalog Number 13601) was compared with Vendor X (in red) and Vendor Y (in green), both of which use Ac-RGK(Ac)-R110 peptide substrate. The signal/background ratio of the HDAC activity measured with Amplite™ Fluorimetric HDAC Activity Assay Kit is more than10 times higher than those of Vendors X and Y.

This document (01.0270.251203r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.

Histone Deacetylases (HDAC)

Amplite® HDAC Activity Assay Kit