Magnetic Beads and Kits

AAT Bioquest's ReadiPrep™ Magnetic Beads and Kits provide streamlined solutions for sample preparation workflows. This product line leverages magnetic separation technology for applications such as rapid, high-purity isolation of target cells, selective removal of dead or apoptotic cells, and affinity purification of His-tagged proteins. Designed for convenience and reproducibility, these products eliminate the need for centrifugation steps and can deliver bead-free final products for downstream applications including flow cytometry, sequencing, and protein analysis.

Product Group	Example Applications
Cell Enrichment	CD4+ T cell purification, live cell enrichment
Protein Purification	His-tagged protein purification
Biomolecule Isolation	Biotinylated molecule, mRNA isolation

Cell Enrichment

ReadiPrep™ Cell Enrichment Kits utilize magnetic bead technology to isolate specific cell populations or remove unwanted cells from samples. These kits deliver bead-free, pure cell populations ready for immediate use in downstream applications such as flow cytometry, single-cell sequencing, and functional assays.

Bead-free final product — isolated cells are released from magnetic beads, ensuring compatibility with sensitive downstream applications
Rapid workflow — complete isolation in under 30 minutes with minimal hands-on time
High purity and recovery — optimized protocols deliver >90% purity with excellent cell viability
No centrifugation required — magnetic separation simplifies processing and reduces cell stress
Reproducible results — standardized reagents ensure consistent performance across experiments

Fig. 1

CD4+ T cells positively isolated from whole blood using ReadiPrep™ CD4+ Cell Isolation Kit. The figure shows that the percentage of CD4+ cells was 36.3% before isolation which increased to 99.38% after isolation. Cells were stained with CD4 (SK3)-iFluor® 488 and CD45 (2D1)-iFlour® 647.

Protein Purification

HIS-tag Protein Purification

ReadiPrep™ Nickel NTA Magnetic Beads provide a fast and simple solution for small-scale affinity purification of histidine-tagged recombinant proteins. The magnetic separation format eliminates the need for gravity flow columns or centrifugation, enabling rapid batch purification with high binding capacity and low non-specific binding.

High binding capacity — efficient capture of His-tagged proteins from crude cell lysates
Fast magnetic separation — eliminates column packing and reduces processing time
Compatible with native and denaturing conditions — flexible purification protocols
Low non-specific binding — proprietary surface chemistry minimizes contaminant co-purification
Scalable format — ideal for small-scale purifications and screening applications

Biomolecule Isolation

Streptavidin Magnetic Beads

ReadiPrep™ Streptavidin Magnetic Beads provide high-affinity capture of biotinylated molecules including antibodies, nucleic acids, and proteins. The streptavidin-biotin interaction (~10⁻¹⁵ M Kd) ensures efficient and stable binding, while uniform magnetic particles enable rapid separation and washing. Multiple bead sizes are available to optimize performance for different applications.

High biotin-binding capacity — approximately 1000 pmol biotin per mg of beads for efficient target capture
Uniform particle sizes — monodisperse beads available in 0.6 μm, 1 μm, and 3 μm diameters
Rapid magnetic separation — larger beads provide faster separation for high-throughput workflows
Versatile applications — ideal for immunoprecipitation, cell isolation, nucleic acid capture, and protein pulldown assays
Low non-specific binding — optimized surface chemistry minimizes background

Oligo(dT) Magnetic Beads for mRNA Isolation

ReadiPrep™ Oligo(dT)30 Magnetic Beads enable rapid and efficient isolation of polyadenylated mRNA from total RNA, cell lysates, or tissue samples. The beads feature covalently coupled oligo(dT)30 sequences that hybridize specifically with the poly(A) tail present on eukaryotic mRNA, allowing selective capture and purification. Isolated mRNA is suitable for downstream applications including cDNA synthesis, RT-PCR, RNA-seq library preparation, and in vitro translation.

Specific mRNA capture — oligo(dT)30 sequences selectively bind poly(A)+ mRNA while excluding rRNA and other non-polyadenylated RNA
High binding capacity — 10 μg mRNA per 100 μL (1 mg) of beads
Fast workflow — complete mRNA isolation in under 30 minutes
Gentle elution — low-temperature elution preserves mRNA integrity
Versatile sample compatibility — works with total RNA, crude cell lysates, and tissue homogenates

This document (01.0339.251203r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.