Protein kinases catalyze the transfer of phosphate groups from ATP to specific substrates, playing critical roles in cell signaling, metabolism, and disease pathways. AAT Bioquest offers protein kinase activity assay kits and associated reagents that measure ATP-to-ADP conversion resulting from substrate phosphorylation, a characteristic outcome of protein kinase reactions, enabling antibody-free and non-radioactive detection of kinase activity under appropriate assay conditions.

Protein kinases transfer phosphate groups from ATP to specific protein substrates, generating ADP as a universal byproduct. Measuring ADP accumulation provides a direct, antibody-free method for detecting any kinase activity. AAT Bioquest offers two complementary assay platforms for universal kinase detection:

Both kits convert ADP to a red fluorescent signal through enzyme-coupled reactions, enabling sensitive and quantitative measurement in microplate format. This approach offers several advantages over traditional methods:

These supporting reagents enable researchers to optimize and control their kinase experiments. Staurosporine is a broad-spectrum kinase inhibitor commonly used as a positive control for kinase inhibition studies and as an apoptosis inducer. The ReadiUse™ Universal Kinase Reaction Stopping Buffer effectively terminates kinase reactions by chelating essential divalent metal ions (Mg²⁺, Mn²⁺), thereby preventing further ATP binding and phosphate transfer, providing consistent endpoint measurements across multiple samples.