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AzoDye-1 PEG10 Succinimidyl Ester
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1113.23 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 522 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| AzoDye-1-PEG10-Azide |
| AzoDye-1 PEG10 Alkyne |
| Overview |  SDSProtocol |
Molecular weight 1113.23 | Absorbance (nm) 522 |
AzoDye-1 PEG10 Succinimidyl Ester is an excellent building block for labeling amino-containing molecules. It contains a PEG10 spacer that significantly improves its water solubility compared to AzoDye-1 succinimidyl ester (#2402) that bears the same extremely hydrophobic BHQ-1 chromophore. AzoDye-1 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-1 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-1's ability to form stable, non-fluorescent complexes with a fluorophore.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-1 PEG10 Succinimidyl Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 89.829 µL | 449.143 µL | 898.287 µL | 4.491 mL | 8.983 mL |
| 5 mM | 17.966 µL | 89.829 µL | 179.657 µL | 898.287 µL | 1.797 mL |
| 10 mM | 8.983 µL | 44.914 µL | 89.829 µL | 449.143 µL | 898.287 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Images
Figure 1. AzoDye-1 PEG10 Succinimidyl Ester is an excellent building block for labeling amino-containing molecules. It contains a PEG10 spacer that significantly improves its water solubility compared to AzoDye-1 succinimidyl ester (#2402) that bears the same extremely hydrophobic BHQ-1 chromophore.