AzoDye-1 PEG10 Alkyne

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Physical properties

Molecular weight	982.14
Solvent	DMSO

Spectral properties

Absorbance (nm)

522

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

AzoDye-1 PEG10 Succinimidyl Ester

AzoDye-1-PEG10-Azide

Overview SDS Protocol

Molecular weight

982.14

Absorbance (nm)

522

AzoDye-1 PEG10 alkyne is an excellent building block for labeling azide-containing molecules. It contains a PEG10 spacer that significantly improves its water solubility compared to AzoDye-1 alkyne (#2414) although both AzoDye-1 alkyne (#2414) and AzoDye-1 PEG10 alkyne (#2415) bear the same extremely hydrophobic BHQ-1 chromophore. AzoDye-1 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-1 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-1's ability to form stable, non-fluorescent complexes with a fluorophore.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-1 PEG10 Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	101.818 µL	509.092 µL	1.018 mL	5.091 mL	10.182 mL
5 mM	20.364 µL	101.818 µL	203.637 µL	1.018 mL	2.036 mL
10 mM	10.182 µL	50.909 µL	101.818 µL	509.092 µL	1.018 mL

Molarity calculator

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Spectrum

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Spectral properties

Absorbance (nm)

522

Images

Figure 1. Chemical structure for AzoDye-1 PEG10 Alkyne.

References

View all 47 references: Citation Explorer

DNA functionalized double quantum dots-based fluorescence biosensor for one-step simultaneous detection of multiple microRNAs.
Authors: Hu, Ou and Li, Zeyu and Tong, Yanli and Wang, Qiyou and Chen, Zuanguang
Journal: Talanta (2021): 122763

Lighting up ATP in cells and tissues using a simple aptamer-based fluorescent probe.
Authors: Liu, Wenjun and Zhu, Xuena and Mozneb, Maedeh and Nagahara, Larry and Hu, Tony Y and Li, Chen-Zhong
Journal: Mikrochimica acta (2021): 352

Detection of Amyloid β Oligomers by a Fluorescence Ratio Strategy Based on Optically Trapped Highly Doped Upconversion Nanoparticles-SiO2@Metal-Organic Framework Microspheres.
Authors: Fang, Wen-Kai and Liu, Liu and Zhang, Li-Ling and Liu, Da and Liu, Yang and Tang, Hong-Wu
Journal: Analytical chemistry (2021): 12447-12455

A fluorescent amplification strategy for high-sensitive detection of 17 β-estradiol based on EXPAR and HCR.
Authors: Wang, Yu and Zhao, Xudong and Zhang, Man and Sun, Xuan and Bai, Jialei and Peng, Yuan and Li, Shuang and Han, Dianpeng and Ren, Shuyue and Wang, Jiang and Han, Tie and Gao, Yifei and Ning, Baoan and Gao, Zhixian
Journal: Analytica chimica acta (2020): 1-8

A Dendron-Based Fluorescence Turn-On Probe for Tumor Detection.
Authors: Liu, Changren and Zhang, Ling'e and Zhou, Sensen and Zhang, Xiaoke and Wu, Wei and Jiang, Xiqun
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 13022-13030

Near-infrared control and real-time detection of osteogenic differentiation in mesenchymal stem cells by multifunctional upconversion nanoparticles.
Authors: Wang, Kaipeng and Wu, Qian and Wang, Xichao and Liang, Guohai and Yang, Anli and Li, Jinming
Journal: Nanoscale (2020): 10106-10116

Preparation of antibody-immobilized gelatin nanospheres incorporating a molecular beacon to visualize the biological function of macrophages.
Authors: Yoshimoto, Yu and Jo, Jun-Ichiro and Tabata, Yasuhiko
Journal: Regenerative therapy (2020): 11-18

Dual Energy Transfer-Based DNA/Graphene Oxide Nanocomplex Probe for Highly Robust and Accurate Monitoring of Apoptosis-Related microRNAs.
Authors: Jiang, Yong Jian and Wang, Na and Cheng, Feng and Lin, Hua Rong and Zhen, Shu Jun and Li, Yuan Fang and Li, Chun Mei and Huang, Cheng Zhi
Journal: Analytical chemistry (2020): 11565-11572

Accurate In-Vivo Quantification of CD19 CAR-T Cells after Treatment with Axicabtagene Ciloleucel (Axi-Cel) and Tisagenlecleucel (Tisa-Cel) Using Digital PCR.
Authors: Badbaran, Anita and Berger, Carolina and Riecken, Kristoffer and Kruchen, Anne and Geffken, Maria and Müller, Ingo and Kröger, Nicolaus and Ayuk, Francis A and Fehse, Boris
Journal: Cancers (2020)

BHQ-Cyanine-Based "Off-On" Long-Circulating Assembly as a Ferroptosis Amplifier for Cancer Treatment: A Lipid-Peroxidation Burst Device.
Authors: Sang, Mangmang and Luo, Renjie and Bai, Yidan and Dou, Jun and Zhang, Zhongtao and Liu, Fulei and Feng, Feng and Liu, Wenyuan
Journal: ACS applied materials & interfaces (2019): 42873-42884