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AzoDye-1-PEG10-Azide

AzoDye-1 PEG10 azide is an excellent building block for labeling alkyne-containing molecules. It contains a PEG10 spacer that significantly improves its water solubility compared to AzoDye-1 azide (#2412) although both AzoDye-1 azide (#2412) and AzoDye-1 PEG10 azide (#2413) bear the same extremely hydrophobic BHQ-1 chromophore. AzoDye-1 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-1 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-1's ability to form stable, non-fluorescent complexes with a fluorophore.

Spectrum

References

View all 50 references: Citation Explorer
DNA functionalized double quantum dots-based fluorescence biosensor for one-step simultaneous detection of multiple microRNAs.
Authors: Hu, Ou and Li, Zeyu and Tong, Yanli and Wang, Qiyou and Chen, Zuanguang
Journal: Talanta (2021): 122763
Lighting up ATP in cells and tissues using a simple aptamer-based fluorescent probe.
Authors: Liu, Wenjun and Zhu, Xuena and Mozneb, Maedeh and Nagahara, Larry and Hu, Tony Y and Li, Chen-Zhong
Journal: Mikrochimica acta (2021): 352
Multifunctional Programmable DNA Nanotrain for Activatable Hypoxia Imaging and Mitochondrion-Targeted Enhanced Photodynamic Therapy.
Authors: Liu, Jin and Ding, Ge and Chen, Shiya and Xue, Caoye and Chen, Mian and Wu, Xu and Yuan, Quan and Zheng, Jing and Yang, Ronghua
Journal: ACS applied materials & interfaces (2021): 9681-9690
A phosphorescence resonance energy transfer-based "off-on" long afterglow aptasensor for cadmium detection in food samples.
Authors: Lai, Bin and Wang, Haitao and Su, Wentao and Wang, Zhouping and Zhu, Bei-Wei and Yu, Chenxu and Tan, Mingqian
Journal: Talanta (2021): 122409
Modification of a SERS-active Ag surface to promote adsorption of charged analytes: effect of Cu2+ ions.
Authors: Ranishenka, Bahdan V and Panarin, Andrei Yu and Chelnokova, Irina A and Terekhov, Sergei N and Mojzes, Peter and Shmanai, Vadim V
Journal: Beilstein journal of nanotechnology (2021): 902-912
Page updated on October 3, 2024

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Catalog Number2413
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Physical properties

Molecular weight

1013.16

Solvent

DMSO

Spectral properties

Absorbance (nm)

522

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501