AzoDye-1-PEG10-Azide
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1013.16 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 522 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
AzoDye-1 PEG10 Succinimidyl Ester |
AzoDye-1 PEG10 Alkyne |
Overview | ![]() ![]() |
See also: Förster Resonance Energy Transfer (FRET)
Molecular weight 1013.16 | Absorbance (nm) 522 |
AzoDye-1 PEG10 azide is an excellent building block for labeling alkyne-containing molecules. It contains a PEG10 spacer that significantly improves its water solubility compared to AzoDye-1 azide (#2412) although both AzoDye-1 azide (#2412) and AzoDye-1 PEG10 azide (#2413) bear the same extremely hydrophobic BHQ-1 chromophore. AzoDye-1 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-1 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-1's ability to form stable, non-fluorescent complexes with a fluorophore.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-1-PEG10-Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 98.701 µL | 493.505 µL | 987.011 µL | 4.935 mL | 9.87 mL |
5 mM | 19.74 µL | 98.701 µL | 197.402 µL | 987.011 µL | 1.974 mL |
10 mM | 9.87 µL | 49.351 µL | 98.701 µL | 493.505 µL | 987.011 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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References
View all 50 references: Citation Explorer
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Modification of a SERS-active Ag surface to promote adsorption of charged analytes: effect of Cu2+ ions.
Authors: Ranishenka, Bahdan V and Panarin, Andrei Yu and Chelnokova, Irina A and Terekhov, Sergei N and Mojzes, Peter and Shmanai, Vadim V
Journal: Beilstein journal of nanotechnology (2021): 902-912
Authors: Ranishenka, Bahdan V and Panarin, Andrei Yu and Chelnokova, Irina A and Terekhov, Sergei N and Mojzes, Peter and Shmanai, Vadim V
Journal: Beilstein journal of nanotechnology (2021): 902-912
High-Sensitivity Sensing of Divalent Copper Ions at the Single Upconversion Nanoparticle Level.
Authors: Wang, Xindong and Zhang, Xiaorong and Huang, Dingxin and Zhao, Tianyu and Zhao, Lili and Fang, Xikui and Yang, Chunhui and Chen, Guanying
Journal: Analytical chemistry (2021): 11686-11691
Authors: Wang, Xindong and Zhang, Xiaorong and Huang, Dingxin and Zhao, Tianyu and Zhao, Lili and Fang, Xikui and Yang, Chunhui and Chen, Guanying
Journal: Analytical chemistry (2021): 11686-11691
A novel method for visualizing and tracking endogenous mRNA in a specific cell population in pathological neovascularization.
Authors: Uddin, Md Imam and Kilburn, Tyler C and Jamal, Sara Z and Duvall, Craig L and Penn, John S
Journal: Scientific reports (2021): 2565
Authors: Uddin, Md Imam and Kilburn, Tyler C and Jamal, Sara Z and Duvall, Craig L and Penn, John S
Journal: Scientific reports (2021): 2565
Near-infrared light excited UCNP-DNAzyme nanosensor for selective detection of Pb2+ and in vivo imaging.
Authors: Huang, Linna and Chen, Feng and Zong, Xia and Lu, Qiujun and Wu, Cuiyan and Ni, Ziqi and Liu, Meiling and Zhang, Youyu
Journal: Talanta (2021): 122156
Authors: Huang, Linna and Chen, Feng and Zong, Xia and Lu, Qiujun and Wu, Cuiyan and Ni, Ziqi and Liu, Meiling and Zhang, Youyu
Journal: Talanta (2021): 122156
Detection of Amyloid β Oligomers by a Fluorescence Ratio Strategy Based on Optically Trapped Highly Doped Upconversion Nanoparticles-SiO2@Metal-Organic Framework Microspheres.
Authors: Fang, Wen-Kai and Liu, Liu and Zhang, Li-Ling and Liu, Da and Liu, Yang and Tang, Hong-Wu
Journal: Analytical chemistry (2021): 12447-12455
Authors: Fang, Wen-Kai and Liu, Liu and Zhang, Li-Ling and Liu, Da and Liu, Yang and Tang, Hong-Wu
Journal: Analytical chemistry (2021): 12447-12455
A fluorescent amplification strategy for high-sensitive detection of 17 β-estradiol based on EXPAR and HCR.
Authors: Wang, Yu and Zhao, Xudong and Zhang, Man and Sun, Xuan and Bai, Jialei and Peng, Yuan and Li, Shuang and Han, Dianpeng and Ren, Shuyue and Wang, Jiang and Han, Tie and Gao, Yifei and Ning, Baoan and Gao, Zhixian
Journal: Analytica chimica acta (2020): 1-8
Authors: Wang, Yu and Zhao, Xudong and Zhang, Man and Sun, Xuan and Bai, Jialei and Peng, Yuan and Li, Shuang and Han, Dianpeng and Ren, Shuyue and Wang, Jiang and Han, Tie and Gao, Yifei and Ning, Baoan and Gao, Zhixian
Journal: Analytica chimica acta (2020): 1-8
Application notes
FAQ
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