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Physical properties
Molecular weight1013.16
Spectral properties
Absorbance (nm)522
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Absorbance (nm)
AzoDye-1 PEG10 azide is an excellent building block for labeling alkyne-containing molecules. It contains a PEG10 spacer that significantly improves its water solubility compared to AzoDye-1 azide (#2412) although both AzoDye-1 azide (#2412) and AzoDye-1 PEG10 azide (#2413) bear the same extremely hydrophobic BHQ-1 chromophore. AzoDye-1 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-1 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-1's ability to form stable, non-fluorescent complexes with a fluorophore.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-1-PEG10-Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM98.701 µL493.505 µL987.011 µL4.935 mL9.87 mL
5 mM19.74 µL98.701 µL197.402 µL987.011 µL1.974 mL
10 mM9.87 µL49.351 µL98.701 µL493.505 µL987.011 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectral properties

Absorbance (nm)522



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