AzoDye-1-PEG10-Azide

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Physical properties

Molecular weight	1013.16
Solvent	DMSO

Spectral properties

Absorbance (nm)

522

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

AzoDye-1 PEG10 Succinimidyl Ester

AzoDye-1 PEG10 Alkyne

Overview SDS Protocol

Molecular weight

1013.16

Absorbance (nm)

522

AzoDye-1 PEG10 azide is an excellent building block for labeling alkyne-containing molecules. It contains a PEG10 spacer that significantly improves its water solubility compared to AzoDye-1 azide (#2412) although both AzoDye-1 azide (#2412) and AzoDye-1 PEG10 azide (#2413) bear the same extremely hydrophobic BHQ-1 chromophore. AzoDye-1 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-1 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-1's ability to form stable, non-fluorescent complexes with a fluorophore.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-1-PEG10-Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	98.701 µL	493.505 µL	987.011 µL	4.935 mL	9.87 mL
5 mM	19.74 µL	98.701 µL	197.402 µL	987.011 µL	1.974 mL
10 mM	9.87 µL	49.351 µL	98.701 µL	493.505 µL	987.011 µL

Molarity calculator

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Spectrum

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Spectral properties

Absorbance (nm)

522

Images

Figure 1. Chemical structure for AzoDye-1-PEG10-Azide.

References

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Authors: Fang, Wen-Kai and Liu, Liu and Zhang, Li-Ling and Liu, Da and Liu, Yang and Tang, Hong-Wu
Journal: Analytical chemistry (2021): 12447-12455

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Journal: Analytica chimica acta (2020): 1-8