AzoDye-2 Succinimidyl Ester
Ordering information
Price | |
Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 603.59 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 554 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
AzoDye-2 Amine |
AzoDye-2 Maleimide |
AzoDye-2 Azide |
AzoDye-2 Alkyne |
Overview | SDSProtocol |
See also: Amine Reactive Dyes and Probes for Conjugation, Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR)
Molecular weight 603.59 | Absorbance (nm) 554 |
AzoDye-2 Succinimidyl Ester is an excellent building block for labeling amino-containing molecules. AzoDye-2 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-2 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-2's ability to form stable, non-fluorescent complexes with a fluorophore.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-2 Succinimidyl Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 165.675 µL | 828.377 µL | 1.657 mL | 8.284 mL | 16.568 mL |
5 mM | 33.135 µL | 165.675 µL | 331.351 µL | 1.657 mL | 3.314 mL |
10 mM | 16.568 µL | 82.838 µL | 165.675 µL | 828.377 µL | 1.657 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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References
View all 50 references: Citation Explorer
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Journal: Analytical chemistry (2020): 5787-5794
Optical Supramolecular Sensing of Creatinine.
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Aptamer-Functionalized Activatable DNA Tetrahedron Nanoprobe for PIWI-Interacting RNA Imaging and Regulating in Cancer Cells.
Authors: Jia, Ruichen and He, Xiaoxiao and Ma, Wenjie and Lei, Yanli and Cheng, Hong and Sun, Huanhuan and Huang, Jin and Wang, Kemin
Journal: Analytical chemistry (2019): 15107-15113
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Journal: Analytical chemistry (2019): 15107-15113
Detection of BCR-ABL1 fusion gene transcripts in the saliva of Nigerian patients with chronic myeloid leukemia.
Authors: Uzoma, I C and Taiwo, I A and Nna, E O and Durosinmi, M A and Ukaejiofo, E O
Journal: Nigerian journal of clinical practice (2019): 51-55
Authors: Uzoma, I C and Taiwo, I A and Nna, E O and Durosinmi, M A and Ukaejiofo, E O
Journal: Nigerian journal of clinical practice (2019): 51-55
Quantum Dot Nanobeacons for Single RNA Labeling and Imaging.
Authors: Ma, Yingxin and Mao, Guobin and Huang, Weiren and Wu, Guoqiang and Yin, Wen and Ji, Xinghu and Deng, Zishi and Cai, Zhiming and Zhang, Xian-En and He, Zhike and Cui, Zongqiang
Journal: Journal of the American Chemical Society (2019): 13454-13458
Authors: Ma, Yingxin and Mao, Guobin and Huang, Weiren and Wu, Guoqiang and Yin, Wen and Ji, Xinghu and Deng, Zishi and Cai, Zhiming and Zhang, Xian-En and He, Zhike and Cui, Zongqiang
Journal: Journal of the American Chemical Society (2019): 13454-13458
A carbon dot and molecular beacon based fluorometric sensor for the cancer marker microRNA-21.
Authors: Mahani, Mohamad and Mousapour, Zhahra and Divsar, Faten and Nomani, Alireza and Ju, Huangxian
Journal: Mikrochimica acta (2019): 132
Authors: Mahani, Mohamad and Mousapour, Zhahra and Divsar, Faten and Nomani, Alireza and Ju, Huangxian
Journal: Mikrochimica acta (2019): 132
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Authors: Fraser, Tamieka A and Bell, Mikaela G and Harris, Patrick N A and Bell, Scott C and Bergh, Haakon and Nguyen, Thuy-Khanh and Kidd, Timothy J and Nimmo, Graeme R and Sarovich, Derek S and Price, Erin P
Journal: Microbial genomics (2019)
Authors: Fraser, Tamieka A and Bell, Mikaela G and Harris, Patrick N A and Bell, Scott C and Bergh, Haakon and Nguyen, Thuy-Khanh and Kidd, Timothy J and Nimmo, Graeme R and Sarovich, Derek S and Price, Erin P
Journal: Microbial genomics (2019)
Correction: Cyclopeptidic photosensitizer prodrugs as proteolytically triggered drug delivery systems of pheophorbide A: part II - co-loading of pheophorbide A and black hole quencher.
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Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2019): 2815
Authors: Bouilloux, Jordan and Yushchenko, Oleksandr and Dereka, Bogdan and Boso, Gianluca and Babič, Andréj and Zbinden, Hugo and Vauthey, Eric and Lange, Norbert
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