Carboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. FITC-labeled nucleotides and peptides tend to deteriorate more quickly than the corresponding FAM conjugates. We found that FAM reagents can be used to substitute FITC reagents in most biological applications.

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Physical properties

Molecular weight	376.32
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	72088-94-9

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] CAS 92557-80-7	493	517	83000	0.32	0.178
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] CAS 92557-81-8	493	517	83000	0.32	0.178
5-FAM ethylenediamine	493	517	83000	0.32	0.178
5-FAM cadaverine	493	517	83000	0.32	0.178
5-FAM Azide	493	517	83000	0.32	0.178
5-FAM Alkyne	493	517	83000	0.32	0.178
6-FAM Azide	493	517	83000	0.32	0.178
6-FAM Alkyne	493	517	83000	0.32	0.178
6-FAM phosphoramidite [5'-Fluorescein phosphoramidite] CAS 204697-37-0	493	517	83000	0.32	0.178
5-FAM Cysteamine	493	517	83000	0.32	0.178
5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]	522	546	94000	0.24	0.214
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] CAS 98181-63-6	552	578	90000	0.32	0.178
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] CAS 198978-94-8	578	604	82000	-	0.168
5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] CAS 95197-95-8	544	570	100000	0.27	0.34
Show More (5)

Citations

View all 6 citations: Citation Explorer

Adjusting Heterodimeric Coiled-Coils (K/E Zipper) to Connect Autophagy-Inducing Peptide with Cell-Penetrating Peptide
Authors: Hakata, Yoshiyuki and Yamashita, Kazuma and Hashimoto, Sonoko and Ohtsuki, Takashi and Miyazawa, Masaaki and Kitamatsu, Mizuki
Journal: Pharmaceutics (2023): 1048

Intracellular delivery of a peptide nucleic acid-based hybrid of an autophagy inducing peptide with a cell-penetrating peptide
Authors: Hakata, Yoshiyuki and Ishikawa, Suzuka and Ohtsuki, Takashi and Miyazawa, Masaaki and Kitamatsu, Mizuki
Journal: Organic \& Biomolecular Chemistry (2020): 1978--1986

Acidic pH-Activated Gas-Generating Nanoparticles with Pullulan Decorating for Hepatoma-Targeted Ultrasound Imaging
Authors: Chen, Shanshan and Xu, Xiao-Lin and Zhou, Boyang and Tian, Jing and Luo, Baoming and Zhang, Li-Ming
Journal: ACS Applied Materials & Interfaces (2019)

Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A
Journal: Advanced Materials (2017)

Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi
Journal: Scientific reports (2015)

References

View all 137 references: Citation Explorer

Carboxyfluorescein leakage from poly(ethylene glycol)-grafted liposomes induced by the interaction with serum
Authors: Hashizaki K, Taguchi H, Sakai H, Abe M, Saito Y, Ogawa N.
Journal: Chem Pharm Bull (Tokyo) (2006): 80

Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (cfse)
Authors: Asquith B, Debacq C, Florins A, Gillet N, Sanchez-Alcaraz T, Mosley A, Willems L.
Journal: Proc Biol Sci (2006): 1165

Low incidence of acute rejection after living-donor liver transplantation: Immunologic analyses by mixed lymphocyte reaction using a carboxyfluorescein diacetate succinimidyl ester labeling technique
Authors: Tanaka Y, Ohdan H, Onoe T, Mitsuta H, Tashiro H, Itamoto T, Asahara T.
Journal: Transplantation (2005): 1262

Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling
Authors: Wang XQ, Duan XM, Liu LH, Fang YQ, Tan Y.
Journal: Acta Biochim Biophys Sin (Shanghai) (2005): 379

Evidence for dimer formation by an amphiphilic heptapeptide that mediates chloride and carboxyfluorescein release from liposomes
Authors: Pajewski R, Ferdani R, Pajewska J, Djedovic N, Schlesinger PH, Gokel GW.
Journal: Org Biomol Chem (2005): 619

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