5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9*
Carboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. FITC-labeled nucleotides and peptides tend to deteriorate more quickly than the corresponding FAM conjugates. We found that FAM reagents can be used to substitute FITC reagents in most biological applications.
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7* | 493 | 517 | 83000 | 0.32 | 0.178 |
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8* | 493 | 517 | 83000 | 0.32 | 0.178 |
5-FAM ethylenediamine | 493 | 517 | 83000 | 0.32 | 0.178 |
5-FAM cadaverine | 493 | 517 | 83000 | 0.32 | 0.178 |
5-FAM Azide | 493 | 517 | 83000 | 0.32 | 0.178 |
5-FAM Alkyne | 493 | 517 | 83000 | 0.32 | 0.178 |
6-FAM Azide | 493 | 517 | 83000 | 0.32 | 0.178 |
6-FAM Alkyne | 493 | 517 | 83000 | 0.32 | 0.178 |
6-FAM phosphoramidite [5'-Fluorescein phosphoramidite] *CAS 204697-37-0* | 493 | 517 | 83000 | 0.32 | 0.178 |
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Citations
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Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A
Journal: Advanced Materials (2017)
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Journal: Advanced Materials (2017)
Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi
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