5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9*

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Chemical structure for 5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9*
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Overview

Ex/Em (nm)494/519
MW376.32
CAS #72088-94-9
SolventDMSO
Storage Freeze (<-15 °C)
Minimize light exposure
Category Peptide Synthesis
Peptide Labeling Reagents
Related Carbonyl-Reactive Biotins
Amine-Reactive Probes
Secondary Reagents
Carboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. FITC-labeled nucleotides and peptides tend to deteriorate more quickly than the corresponding FAM conjugates. We found that FAM reagents can be used to substitute FITC reagents in most biological applications.




Calculators
Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



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Mass Molecular weight Volume Concentration Moles
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References & Citations

Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Christopher K Arakawa, Barry A Badeau, Ying Zheng, Cole A DeForest
Journal: Advanced Materials (2017)

Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Yu-Hsuan Peng, Douglas H Sweet, Shiuan-Pey Lin, Chung-Ping Yu, Pei-Dawn Lee Chao, Yu-Chi Hou
Journal: Scientific reports (2015)

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Søren Preus, L Marcus Wilhelmsson
Journal: ChemBioChem (2012): 1990--2001






Additional Documents

 
Safety Data Sheet (SDS)


Catalogs
1. Oligonucleotide Labeling Reagents
2. Peptide Labeling Reagents

Certificate of Analysis