5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] *CAS 117548-22-8*
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Physical properties
Molecular weight | 473.39 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.178 |
Extinction coefficient (cm -1 M -1) | 83000 |
Excitation (nm) | 493 |
Emission (nm) | 517 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
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See also: Amine Reactive Dyes and Probes for Conjugation, Bioconjugation, Cell Structures and Organelles, Nucleus, Chemical Reagents, Classic Dyes, Dyes by Functional Group, Digital PCR, DNA and RNA Quantitation, Fluoresceins, Physiological Probes, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Purification & Analysis, PCR Detection of Viral DNA/RNA
CAS 117548-22-8 | Molecular weight 473.39 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.178 | Extinction coefficient (cm -1 M -1) 83000 | Excitation (nm) 493 | Emission (nm) 517 |
Carboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. FITC-labeled nucleotides and peptides tend to deteriorate more quickly than the corresponding FAM conjugates. We found that FAM reagents can be used to substitute FITC reagents in most biological applications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] *CAS 117548-22-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 211.242 µL | 1.056 mL | 2.112 mL | 10.562 mL | 21.124 mL |
5 mM | 42.248 µL | 211.242 µL | 422.485 µL | 2.112 mL | 4.225 mL |
10 mM | 21.124 µL | 105.621 µL | 211.242 µL | 1.056 mL | 2.112 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.178 |
Extinction coefficient (cm -1 M -1) | 83000 |
Excitation (nm) | 493 |
Emission (nm) | 517 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
5(6)-FAM ethylenediamine | 493 | 517 | 83000 | 0.32 | 0.178 |
5(6)-FAM cadaverine | 493 | 517 | 83000 | 0.32 | 0.178 |
5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers* | 522 | 546 | 94000 | 0.24 | 0.214 |
5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *CAS 246256-50-8* | 552 | 578 | 90000 | 0.32 | 0.178 |
5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] *Mixed isomers* | 578 | 604 | 82000 | - | 0.168 |
Images
Citations
View all 3 citations: Citation Explorer
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Authors: Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A
Journal: Advanced Materials (2017)
Authors: Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A
Journal: Advanced Materials (2017)
Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi
Journal: Scientific reports (2015)
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi
Journal: Scientific reports (2015)
Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001
References
View all 137 references: Citation Explorer
Carboxyfluorescein leakage from poly(ethylene glycol)-grafted liposomes induced by the interaction with serum
Authors: Hashizaki K, Taguchi H, Sakai H, Abe M, Saito Y, Ogawa N.
Journal: Chem Pharm Bull (Tokyo) (2006): 80
Authors: Hashizaki K, Taguchi H, Sakai H, Abe M, Saito Y, Ogawa N.
Journal: Chem Pharm Bull (Tokyo) (2006): 80
Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (cfse)
Authors: Asquith B, Debacq C, Florins A, Gillet N, Sanchez-Alcaraz T, Mosley A, Willems L.
Journal: Proc Biol Sci (2006): 1165
Authors: Asquith B, Debacq C, Florins A, Gillet N, Sanchez-Alcaraz T, Mosley A, Willems L.
Journal: Proc Biol Sci (2006): 1165
Application of alamar blue/5-carboxyfluorescein diacetate acetoxymethyl ester as a noninvasive cell viability assay in primary hepatocytes from rainbow trout
Authors: Schreer A, Tinson C, Sherry JP, Schirmer K.
Journal: Anal Biochem (2005): 76
Authors: Schreer A, Tinson C, Sherry JP, Schirmer K.
Journal: Anal Biochem (2005): 76
Low incidence of acute rejection after living-donor liver transplantation: Immunologic analyses by mixed lymphocyte reaction using a carboxyfluorescein diacetate succinimidyl ester labeling technique
Authors: Tanaka Y, Ohdan H, Onoe T, Mitsuta H, Tashiro H, Itamoto T, Asahara T.
Journal: Transplantation (2005): 1262
Authors: Tanaka Y, Ohdan H, Onoe T, Mitsuta H, Tashiro H, Itamoto T, Asahara T.
Journal: Transplantation (2005): 1262
Evidence for dimer formation by an amphiphilic heptapeptide that mediates chloride and carboxyfluorescein release from liposomes
Authors: Pajewski R, Ferdani R, Pajewska J, Djedovic N, Schlesinger PH, Gokel GW.
Journal: Org Biomol Chem (2005): 619
Authors: Pajewski R, Ferdani R, Pajewska J, Djedovic N, Schlesinger PH, Gokel GW.
Journal: Org Biomol Chem (2005): 619
Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling
Authors: Wang XQ, Duan XM, Liu LH, Fang YQ, Tan Y.
Journal: Acta Biochim Biophys Sin (Shanghai) (2005): 379
Authors: Wang XQ, Duan XM, Liu LH, Fang YQ, Tan Y.
Journal: Acta Biochim Biophys Sin (Shanghai) (2005): 379
Application of glutaraldehyde for the staining of esterase-active cells with carboxyfluorescein diacetate
Authors: Morono Y, Takano S, Miyanaga K, Tanji Y, Unno H, Hori K.
Journal: Biotechnol Lett (2004): 379
Authors: Morono Y, Takano S, Miyanaga K, Tanji Y, Unno H, Hori K.
Journal: Biotechnol Lett (2004): 379
Drug efflux transport properties of 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (bcecf-am) and its fluorescent free acid, bcecf
Authors: Bachmeier CJ, Trickler WJ, Miller DW.
Journal: J Pharm Sci (2004): 932
Authors: Bachmeier CJ, Trickler WJ, Miller DW.
Journal: J Pharm Sci (2004): 932
pruning of alloreactive cd4+ t cells using 5- (and 6-)carboxyfluorescein diacetate succinimidyl ester prolongs skin allograft survival
Authors: Watson D, Zhang GY, Sartor M, Alex and er SI., undefined
Journal: J Immunol (2004): 6574
Authors: Watson D, Zhang GY, Sartor M, Alex and er SI., undefined
Journal: J Immunol (2004): 6574
Cyclodextrin enhanced transdermal delivery of piroxicam and carboxyfluorescein by electroporation
Authors: Murthy SN, Zhao YL, Sen A, Hui SW.
Journal: J Control Release (2004): 393
Authors: Murthy SN, Zhao YL, Sen A, Hui SW.
Journal: J Control Release (2004): 393
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)