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5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] *CAS 117548-22-8*
Carboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. FITC-labeled nucleotides and peptides tend to deteriorate more quickly than the corresponding FAM conjugates. We found that FAM reagents can be used to substitute FITC reagents in most biological applications.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] *CAS 117548-22-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 211.242 µL | 1.056 mL | 2.112 mL | 10.562 mL | 21.124 mL |
| 5 mM | 42.248 µL | 211.242 µL | 422.485 µL | 2.112 mL | 4.225 mL |
| 10 mM | 21.124 µL | 105.621 µL | 211.242 µL | 1.056 mL | 2.112 mL |
Molarity calculator
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| 5(6)-FAM cadaverine | 493 | 517 | 83000 | 0.32 | 0.178 |
| 5(6)-FAM ethylenediamine | 493 | 517 | 83000 | 0.32 | 0.178 |
| 5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers* | 522 | 546 | 94000 | 0.24 | 0.214 |
| 5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] *Mixed isomers* | 578 | 604 | 82000 | - | 0.168 |
| 5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *CAS 246256-50-8* | 552 | 578 | 90000 | 0.32 | 0.178 |
Citations
View all 3 citations: Citation Explorer
Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A
Journal: Advanced Materials (2017)
Authors: Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A
Journal: Advanced Materials (2017)
Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi
Journal: Scientific reports (2015)
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi
Journal: Scientific reports (2015)
Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001
References
View all 137 references: Citation Explorer
Carboxyfluorescein leakage from poly(ethylene glycol)-grafted liposomes induced by the interaction with serum
Authors: Hashizaki K, Taguchi H, Sakai H, Abe M, Saito Y, Ogawa N.
Journal: Chem Pharm Bull (Tokyo) (2006): 80
Authors: Hashizaki K, Taguchi H, Sakai H, Abe M, Saito Y, Ogawa N.
Journal: Chem Pharm Bull (Tokyo) (2006): 80
Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (cfse)
Authors: Asquith B, Debacq C, Florins A, Gillet N, Sanchez-Alcaraz T, Mosley A, Willems L.
Journal: Proc Biol Sci (2006): 1165
Authors: Asquith B, Debacq C, Florins A, Gillet N, Sanchez-Alcaraz T, Mosley A, Willems L.
Journal: Proc Biol Sci (2006): 1165
Evidence for dimer formation by an amphiphilic heptapeptide that mediates chloride and carboxyfluorescein release from liposomes
Authors: Pajewski R, Ferdani R, Pajewska J, Djedovic N, Schlesinger PH, Gokel GW.
Journal: Org Biomol Chem (2005): 619
Authors: Pajewski R, Ferdani R, Pajewska J, Djedovic N, Schlesinger PH, Gokel GW.
Journal: Org Biomol Chem (2005): 619
Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling
Authors: Wang XQ, Duan XM, Liu LH, Fang YQ, Tan Y.
Journal: Acta Biochim Biophys Sin (Shanghai) (2005): 379
Authors: Wang XQ, Duan XM, Liu LH, Fang YQ, Tan Y.
Journal: Acta Biochim Biophys Sin (Shanghai) (2005): 379
Low incidence of acute rejection after living-donor liver transplantation: Immunologic analyses by mixed lymphocyte reaction using a carboxyfluorescein diacetate succinimidyl ester labeling technique
Authors: Tanaka Y, Ohdan H, Onoe T, Mitsuta H, Tashiro H, Itamoto T, Asahara T.
Journal: Transplantation (2005): 1262
Authors: Tanaka Y, Ohdan H, Onoe T, Mitsuta H, Tashiro H, Itamoto T, Asahara T.
Journal: Transplantation (2005): 1262
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