5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] *CAS 117548-22-8*

5(6)-FAM, SE

5-(and-6)-Carboxyfluorescein, succinimidyl ester; CAS 117548-22-8

Carboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. FITC-labeled nucleotides and peptides tend to deteriorate more quickly than the corresponding FAM conjugates. We found that FAM reagents can be used to substitute FITC reagents in most biological applications.

Protocol

COA

Catalog	Size	Price
110	25 mg	Price
111	100 mg	Price
112	1 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] *CAS 117548-22-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	211.242 µL	1.056 mL	2.112 mL	10.562 mL	21.124 mL
5 mM	42.248 µL	211.242 µL	422.485 µL	2.112 mL	4.225 mL
10 mM	21.124 µL	105.621 µL	211.242 µL	1.056 mL	2.112 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	473.39 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation

Authors:

Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A

Journal:

Advanced Materials (2017)

Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure

Authors:

Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi

Journal:

Scientific reports (2015)

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids

Authors:

Preus, Søren and Wilhelmsson, L Marcus

Journal:

ChemBioChem (2012): 1990--2001

References

View all 137 references: Citation Explorer

Carboxyfluorescein leakage from poly(ethylene glycol)-grafted liposomes induced by the interaction with serum

Authors:

Hashizaki K, Taguchi H, Sakai H, Abe M, Saito Y, Ogawa N.

Journal:

Chem Pharm Bull (Tokyo) (2006): 80

Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (cfse)

Authors:

Asquith B, Debacq C, Florins A, Gillet N, Sanchez-Alcaraz T, Mosley A, Willems L.

Journal:

Proc Biol Sci (2006): 1165

Evidence for dimer formation by an amphiphilic heptapeptide that mediates chloride and carboxyfluorescein release from liposomes

Authors:

Pajewski R, Ferdani R, Pajewska J, Djedovic N, Schlesinger PH, Gokel GW.

Journal:

Org Biomol Chem (2005): 619

Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling

Authors:

Wang XQ, Duan XM, Liu LH, Fang YQ, Tan Y.

Journal:

Acta Biochim Biophys Sin (Shanghai) (2005): 379

Low incidence of acute rejection after living-donor liver transplantation: Immunologic analyses by mixed lymphocyte reaction using a carboxyfluorescein diacetate succinimidyl ester labeling technique

Authors:

Tanaka Y, Ohdan H, Onoe T, Mitsuta H, Tashiro H, Itamoto T, Asahara T.

Journal:

Transplantation (2005): 1262

Physical properties

Molecular weight	473.39
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	117548-22-8

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 30, 2025