5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] CAS 92557-80-7

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Physical properties

Molecular weight	473.39
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

92557-80-7

Molecular weight

473.39

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm ^-1 M ^-1)

83000

Excitation (nm)

493

Emission (nm)

517

5-FAM, SE is the amine-reactive succinimidyl ester of single isomer 5-FAM acid. It is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	211.242 µL	1.056 mL	2.112 mL	10.562 mL	21.124 mL
5 mM	42.248 µL	211.242 µL	422.485 µL	2.112 mL	4.225 mL
10 mM	21.124 µL	105.621 µL	211.242 µL	1.056 mL	2.112 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5(6)-FAM [5-(and-6)-Carboxyfluorescein] CAS 72088-94-9	493	517	83000	0.32	0.178
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] CAS 92557-81-8	493	517	83000	0.32	0.178
5-FAM ethylenediamine	493	517	83000	0.32	0.178
5-FAM cadaverine	493	517	83000	0.32	0.178
5-FAM Azide	493	517	83000	0.32	0.178
5-FAM Alkyne	493	517	83000	0.32	0.178
5-CR6G, SE [5-Carboxyrhodamine 6G, succinimidyl ester] Single isomer	522	546	94000	0.24	0.214
5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] CAS#: 150810-68-7	552	578	90000	0.32	0.178
5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] CAS 209734-74-7	578	604	82000	-	0.168

Images

Figure 1. Chemical structure for 5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7*

Citations

View all 4 citations: Citation Explorer

G9A promotes gastric cancer metastasis by upregulating ITGB3 in a SET domain-independent manner
Authors: Hu, Lei and Wang, He-xiao and Zhang, Bao-gui and Wang, Zhen-qiang and Fan, Zhi-yuan and Wu, Huo and Li, Jian-fang and Su, Li-ping and Yan, Min and Zhu, Zhi-qiang and others, undefined
Journal: Cell death & disease (2018): 278

Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A
Journal: Advanced Materials (2017)

Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi
Journal: Scientific reports (2015)

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

References

View all 137 references: Citation Explorer

Carboxyfluorescein leakage from poly(ethylene glycol)-grafted liposomes induced by the interaction with serum
Authors: Hashizaki K, Taguchi H, Sakai H, Abe M, Saito Y, Ogawa N.
Journal: Chem Pharm Bull (Tokyo) (2006): 80

Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (cfse)
Authors: Asquith B, Debacq C, Florins A, Gillet N, Sanchez-Alcaraz T, Mosley A, Willems L.
Journal: Proc Biol Sci (2006): 1165

Application of alamar blue/5-carboxyfluorescein diacetate acetoxymethyl ester as a noninvasive cell viability assay in primary hepatocytes from rainbow trout
Authors: Schreer A, Tinson C, Sherry JP, Schirmer K.
Journal: Anal Biochem (2005): 76

Low incidence of acute rejection after living-donor liver transplantation: Immunologic analyses by mixed lymphocyte reaction using a carboxyfluorescein diacetate succinimidyl ester labeling technique
Authors: Tanaka Y, Ohdan H, Onoe T, Mitsuta H, Tashiro H, Itamoto T, Asahara T.
Journal: Transplantation (2005): 1262

Evidence for dimer formation by an amphiphilic heptapeptide that mediates chloride and carboxyfluorescein release from liposomes
Authors: Pajewski R, Ferdani R, Pajewska J, Djedovic N, Schlesinger PH, Gokel GW.
Journal: Org Biomol Chem (2005): 619

Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling
Authors: Wang XQ, Duan XM, Liu LH, Fang YQ, Tan Y.
Journal: Acta Biochim Biophys Sin (Shanghai) (2005): 379

Application of glutaraldehyde for the staining of esterase-active cells with carboxyfluorescein diacetate
Authors: Morono Y, Takano S, Miyanaga K, Tanji Y, Unno H, Hori K.
Journal: Biotechnol Lett (2004): 379

Drug efflux transport properties of 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (bcecf-am) and its fluorescent free acid, bcecf
Authors: Bachmeier CJ, Trickler WJ, Miller DW.
Journal: J Pharm Sci (2004): 932

pruning of alloreactive cd4+ t cells using 5- (and 6-)carboxyfluorescein diacetate succinimidyl ester prolongs skin allograft survival
Authors: Watson D, Zhang GY, Sartor M, Alex and er SI., undefined
Journal: J Immunol (2004): 6574

Cyclodextrin enhanced transdermal delivery of piroxicam and carboxyfluorescein by electroporation
Authors: Murthy SN, Zhao YL, Sen A, Hui SW.
Journal: J Control Release (2004): 393