Name: 5-FAM-YVAD-FMK
Brand: AAT Bioquest
SKU: 13473
Availability: InStock

5-FAM-YVAD-FMK

FAM-YVAD-FMK, i.e., 5-carboxyfluorescein-Tyr-Val-Ala-Asp-fluoromethylketone, binds irreversibly to active caspase 1 in stimulated cells. The fluorescent intensity of the FAM-YVAD-FMK signal is proportional to the amount of active caspase 1 and can be easily detected at by fluorescence microscopy, flow cytometer, or fluorescent microplate reader.

Protocol

COA

Catalog	Size	Price	Quantity
13473	25 Tests	Price
13483	100 Tests	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-FAM-YVAD-FMK to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	116.981 µL	584.905 µL	1.17 mL	5.849 mL	11.698 mL
5 mM	23.396 µL	116.981 µL	233.962 µL	1.17 mL	2.34 mL
10 mM	11.698 µL	58.49 µL	116.981 µL	584.905 µL	1.17 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	854.84 g/mol	=	mL	x	mM	=	mmol

Citations

View all 30 citations: Citation Explorer

Diosgenin inhibited podocyte pyroptosis in diabetic kidney disease by regulating the Nrf2/NLRP3 pathway

Authors:

TANG, YU and HU, WENXIAO and PENG, YAJUN and LING, XIANGDONG

Journal:

Biocell (2024): 1503--1516

The Dietary Flavonoid Fisetin Causes Cell Cycle Arrest, Caspase-Dependent Apoptosis, and Enhanced Cytotoxicity of Chemotherapeutic Drugs in Triple-Negative Breast Cancer Cells

Authors:

Smith, M. L., Murphy, K., Doucette, C. D., Greenshields, A. L., Hoskin, D. W.

Journal:

J Cell Biochem (2016): 1913-25

Contribution of membrane progesterone receptor alpha to the induction of progesterone-mediated apoptosis associated with mitochondrial membrane disruption and caspase cascade activation in Jurkat cell lines

Authors:

Kon, A., Yuan, B., Hanazawa, T., Kikuchi, H., Sato, M., Furutani, R., Takagi, N., Toyoda, H.

Journal:

Oncol Rep (2013): 1965-70

Pan-caspase inhibition suppresses polyethylene particle-induced osteolysis

Authors:

L, undefined and graeber, S., Jaeckel, S., Loer, F., Wedemeyer, C., Hilken, G., Canbay, A., Totsch, M., von Knoch, M.

Journal:

Apoptosis (2009): 173-81

Inhibition of Caspase-Like Activities Prevents the Appearance of Reactive Oxygen Species and Dark-Induced Apoptosis in the Unicellular Chlorophyte Dunaliella Tertiolecta(1)

Authors:

Segovia, M., Berges, J. A.

Journal:

J Phycol (2009): 1116-26

References

View all 26 references: Citation Explorer

Suppression of human T cell proliferation by the caspase inhibitors, z-VAD-FMK and z-IETD-FMK is independent of their caspase inhibition properties

Authors:

Lawrence CP, Chow SC.

Journal:

Toxicol Appl Pharmacol. (2012)

Inhibition of elicitation of allergic contact dermatitis by topical use of Z-VAD-FMK, a broad caspase inhibitor: experiment in mice

Authors:

Li YY, Yan CL.

Journal:

Zhonghua Yi Xue Za Zhi (2012): 1992

Structure of human caspase-6 in complex with Z-VAD-FMK: New peptide binding mode observed for the non-canonical caspase conformation

Authors:

Muller I, Lamers MB, Ritchie AJ, Dominguez C, Munoz-Sanjuan I, Kiselyov A.

Journal:

Bioorg Med Chem Lett (2011): 5244

Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss

Authors:

Abaamrane L, Raffin F, Schmerber S, Sendowski I.

Journal:

Eur Arch Otorhinolaryngol (2011): 987

Plasmodium falciparum metacaspase PfMCA-1 triggers a z-VAD-fmk inhibitable protease to promote cell death

Authors:

Meslin B, Beavogui AH, Fasel N, Picot S.

Journal:

PLoS One (2011): e23867

Physical properties

Molecular weight	854.84
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet (Catalog 13473)
Safety Data Sheet (Catalog 13483)
Certificate of Analysis

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Page updated on October 30, 2025