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5-TAMRA C6 maleimide
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Application notes
5-TAMRA C6 maleimide is an excellent replacement for tetramethylrhodamine-5-maleimide. It selectively reacts with thiol groups of peptides, proteins and other biological molecules (such as thiol-modified oligonucleotides and small thiol-containing organic molecules). It might give higher conjugation yield than tetramethylrhodamine-5-maleimide under similar conjugation conditions. 5-TMR C6 maleimide has the spectral propertied identical to those of tetramethylrhodamine-5-maleimide, thus all the instrument settings of the tetramethylrhodamine-5-maleimide would be applicable to the applications of 5-TMR C6 maleimide conjugates.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-TAMRA C6 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 160.341 µL | 801.706 µL | 1.603 mL | 8.017 mL | 16.034 mL |
| 5 mM | 32.068 µL | 160.341 µL | 320.682 µL | 1.603 mL | 3.207 mL |
| 10 mM | 16.034 µL | 80.171 µL | 160.341 µL | 801.706 µL | 1.603 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| 5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] *Mixed isomers* | 552 | 578 | 90000 | 0.32 | 0.178 |
| 6-TAMRA C6 maleimide | 552 | 578 | 90000 | 0.32 | 0.178 |
Citations
View all 2 citations: Citation Explorer
Isolation of a human SARS-CoV-2 neutralizing antibody from a synthetic phage library and its conversion to fluorescent biosensors
Authors: Li, Haimei and Zhu, Bo and Li, Baowei and Chen, Limei and Ning, Xuerao and Dong, Hang and Liang, Jingru and Yang, Xueying and Dong, Jinhua and Ueda, Hiroshi
Journal: Scientific reports (2022): 1--14
Authors: Li, Haimei and Zhu, Bo and Li, Baowei and Chen, Limei and Ning, Xuerao and Dong, Hang and Liang, Jingru and Yang, Xueying and Dong, Jinhua and Ueda, Hiroshi
Journal: Scientific reports (2022): 1--14
Interchromophoric Interactions Between TMR, Alexa, and BODIPY Fluorophores
Authors: Donaphon, Bryan M
Journal: (2018)
Authors: Donaphon, Bryan M
Journal: (2018)
References
View all 21 references: Citation Explorer
Severing of F-actin by yeast cofilin is pH-independent
Authors: Pavlov D, Muhlrad A, Cooper J, Wear M, Reisler E.
Journal: Cell Motil Cytoskeleton (2006): 533
Authors: Pavlov D, Muhlrad A, Cooper J, Wear M, Reisler E.
Journal: Cell Motil Cytoskeleton (2006): 533
Fluorometric measurements of intermolecular distances between the alpha and beta subunits of the Na+/K+-ATPase
Authors: Dempski RE, Hartung K, Friedrich T, Bamberg E.
Journal: J Biol Chem. (2006)
Authors: Dempski RE, Hartung K, Friedrich T, Bamberg E.
Journal: J Biol Chem. (2006)
4-aminopyridine prevents the conformational changes associated with P/C-type inactivation in Shaker channels
Authors: Claydon TW, Vaid M, Rezazadeh S, Kehl SJ, Fedida D.
Journal: J Pharmacol Exp Ther. (2006)
Authors: Claydon TW, Vaid M, Rezazadeh S, Kehl SJ, Fedida D.
Journal: J Pharmacol Exp Ther. (2006)
Both rotor and stator subunits are necessary for efficient binding of F1 to F0 in functionally assembled Escherichia coli ATP synthase
Authors: Krebstakies T, Zimmermann B, Graber P, Altendorf K, Borsch M, Greie JC.
Journal: J Biol Chem (2005): 33338
Authors: Krebstakies T, Zimmermann B, Graber P, Altendorf K, Borsch M, Greie JC.
Journal: J Biol Chem (2005): 33338
The beta subunit of the Na+/K+-ATPase follows the conformational state of the holoenzyme
Authors: Dempski RE, Friedrich T, Bamberg E.
Journal: J Gen Physiol (2005): 505
Authors: Dempski RE, Friedrich T, Bamberg E.
Journal: J Gen Physiol (2005): 505