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7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 150321-92-9*
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Physical properties
| Molecular weight | 317.25 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.171 |
| Excitation (nm) | 355 |
| Emission (nm) | 405 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
CAS 150321-92-9 | Molecular weight 317.25 | Correction Factor (280 nm) 0.171 | Excitation (nm) 355 | Emission (nm) 405 |
7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester is an excellent amino-reactive tag that has strong blue fluorescence. It is used to label peptides, nucleotides. It is also used to label cell membranes although its fluorescence wavelength is quite short.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 150321-92-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 315.209 µL | 1.576 mL | 3.152 mL | 15.76 mL | 31.521 mL |
| 5 mM | 63.042 µL | 315.209 µL | 630.418 µL | 3.152 mL | 6.304 mL |
| 10 mM | 31.521 µL | 157.604 µL | 315.209 µL | 1.576 mL | 3.152 mL |
Molarity calculator
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
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Spectral properties
| Correction Factor (280 nm) | 0.171 |
| Excitation (nm) | 355 |
| Emission (nm) | 405 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Correction Factor (280 nm) |
| 7-Hydroxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 134471-24-2* | 352 | 407 | 0.0815 |
Images
Figure 1. Chemical structure for 7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 150321-92-9*
References
View all 3 references: Citation Explorer
Synthesis of N epsilon-(7-diethylaminocoumarin-3-carboxyl)- and N epsilon-(7-methoxycoumarin-3-carboxyl)-L-Fmoc lysine as tools for protease cleavage detection by fluorescence
Authors: Berthelot T, Talbot JC, Lain G, Deleris G, Latxague L.
Journal: J Pept Sci (2005): 153
Authors: Berthelot T, Talbot JC, Lain G, Deleris G, Latxague L.
Journal: J Pept Sci (2005): 153
An alternative quenched fluorescence substrate for Pz-peptidase
Authors: Tisljar U, et al.
Journal: Anal Biochem (1990): 112-5
Authors: Tisljar U, et al.
Journal: Anal Biochem (1990): 112-5
Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131