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6-FAM phosphoramidite
5'-Fluorescein phosphoramidite; CAS 204697-37-0
There are several ways of labeling an oligonucleotide with fluorescein. The choice of label is diversified further, depending on the spectral requirements. 5'-fluorescein-CE phosphoramidite, derived from the single isomer 6-carboxyfluorescein, 5'-hexachlorofluorescein-CE phosphoramidite (HEX) and 5'-tetrachlorofluorescein-CE phosphoramidite (TET) can all be used to efficiently label an oligonucleotide at the 5'-end. Labeling the 3'-end of an oligo with fluorescein can be achieved using 3'-Fluorescein CPGs. Standard cleavage and deprotection with ammonium hydroxide liberates the fluorescein-labeled oligo when using any of these supports.
Chemical structure for 6-FAM phosphoramidite [5'-Fluorescein phosphoramidite] *CAS 204697-37-0*
Chemical structure for 6-FAM phosphoramidite [5'-Fluorescein phosphoramidite] *CAS 204697-37-0*
CatalogSize
Price
Quantity
6016100 umoles
Price
601710x100 umoles
Price
 
Physical properties

Molecular weight843.94
SolventMeCN
Spectral properties

Correction factor (260 nm)0.32
Correction factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)
83000
Excitation (nm)493
Emission (nm)517
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
CAS204697-37-0
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Page updated on October 4, 2025