6-JOE acid

6-JOE has similar absorption and emission spectra to those of rhodamine 6G, and its SE is predominantly used in automated DNA sequencing. It is also used to label nucleotides. 6-JOE acid is the most economic form to make a conjugate with amino-containing biomolecules.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
204	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-JOE acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	197.918 µL	989.59 µL	1.979 mL	9.896 mL	19.792 mL
5 mM	39.584 µL	197.918 µL	395.836 µL	1.979 mL	3.958 mL
10 mM	19.792 µL	98.959 µL	197.918 µL	989.59 µL	1.979 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	505.26 g/mol	=	mL	x	mM	=	mmol

References

View all 4 references: Citation Explorer

Optical Biosensing System for the Detection of Survivin mRNA in Colorectal Cancer Cells Using a Graphene Oxide Carrier-Bound Oligonucleotide Molecular Beacon.

Authors:

Ratajczak, Katarzyna and Krazinski, Bartlomiej E and Kowalczyk, Anna E and Dworakowska, Beata and Jakiela, Slawomir and Stobiecka, Magdalena

Journal:

Nanomaterials (Basel, Switzerland) (2018)

Hairpin-Hairpin Molecular Beacon Interactions for Detection of Survivin mRNA in Malignant SW480 Cells.

Authors:

Ratajczak, Katarzyna and Krazinski, Bartlomiej E and Kowalczyk, Anna E and Dworakowska, Beata and Jakiela, Slawomir and Stobiecka, Magdalena

Journal:

ACS applied materials & interfaces (2018): 17028-17039

Molecular beacons with JOE dye: Influence of linker and 3' couple quencher.

Authors:

Tsybulsky, Dmitry A and Kvach, Maksim V and Ryazantsev, Dmitry Y and Aparin, Ilya O and Stakheev, Alexander A and Prokhorenko, Igor A and Martynenko, Yury V and Gontarev, Sergey V and Formanovsky, Andrey A and Zatsepin, Timofei S and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K

Journal:

Molecular and cellular probes (2016): 285-290

Short PNA molecular beacons for real-time PCR allelic discrimination of single nucleotide polymorphisms.

Authors:

Petersen, Kenneth and Vogel, Ulla and Rockenbauer, Eszter and Nielsen, Kirsten Vang and Kølvraa, Steen and Bolund, Lars and Nexø, Bjørn

Journal:

Molecular and cellular probes (2004): 117-22

Physical properties

Molecular weight	505.26
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	520
Emission (nm)	545

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 20, 2025