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6-JOE acid
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 505.26 |
| Solvent | DMSO |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 750001 |
| Excitation (nm) | 520 |
| Emission (nm) | 545 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| 6-JOE azide |
| 6-JOE alkyne |
| Overview |  SDSProtocol |
Molecular weight 505.26 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 520 | Emission (nm) 545 |
6-JOE has similar absorption and emission spectra to those of rhodamine 6G, and its SE is predominantly used in automated DNA sequencing. It is also used to label nucleotides. 6-JOE acid is the most economic form to make a conjugate with amino-containing biomolecules.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-JOE acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 197.918 µL | 989.59 µL | 1.979 mL | 9.896 mL | 19.792 mL |
| 5 mM | 39.584 µL | 197.918 µL | 395.836 µL | 1.979 mL | 3.958 mL |
| 10 mM | 19.792 µL | 98.959 µL | 197.918 µL | 989.59 µL | 1.979 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 750001 |
| Excitation (nm) | 520 |
| Emission (nm) | 545 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3* | 520 | 545 | 750001 | - |
| 6-OG488 acid [equivalent to Oregon Green® 488 carboxylic acid, 6-isomer] | 498 | 526 | 76000 | 0.12 |
| 6-JOE Phosphoramidite | 520 | 545 | 750001 | - |
| 6-ROXtra™ acid | 578 | 595 | - | - |
| 6-HEX acid | 533 | 559 | 73000 | 0.15 |
| 6-TET Acid | 521 | 542 | 73000 | 0.13 |
Images
Figure 1. 6-JOE has similar absorption and emission spectra to those of rhodamine 6G, and its SE is predominantly used in automated DNA sequencing. It is also used to label nucleotides. 6-JOE acid is the most economic form to make a conjugate with amino-containing biomolecules.
References
View all 4 references: Citation Explorer
Hairpin-Hairpin Molecular Beacon Interactions for Detection of Survivin mRNA in Malignant SW480 Cells.
Authors: Ratajczak, Katarzyna and Krazinski, Bartlomiej E and Kowalczyk, Anna E and Dworakowska, Beata and Jakiela, Slawomir and Stobiecka, Magdalena
Journal: ACS applied materials & interfaces (2018): 17028-17039
Authors: Ratajczak, Katarzyna and Krazinski, Bartlomiej E and Kowalczyk, Anna E and Dworakowska, Beata and Jakiela, Slawomir and Stobiecka, Magdalena
Journal: ACS applied materials & interfaces (2018): 17028-17039
Optical Biosensing System for the Detection of Survivin mRNA in Colorectal Cancer Cells Using a Graphene Oxide Carrier-Bound Oligonucleotide Molecular Beacon.
Authors: Ratajczak, Katarzyna and Krazinski, Bartlomiej E and Kowalczyk, Anna E and Dworakowska, Beata and Jakiela, Slawomir and Stobiecka, Magdalena
Journal: Nanomaterials (Basel, Switzerland) (2018)
Authors: Ratajczak, Katarzyna and Krazinski, Bartlomiej E and Kowalczyk, Anna E and Dworakowska, Beata and Jakiela, Slawomir and Stobiecka, Magdalena
Journal: Nanomaterials (Basel, Switzerland) (2018)
Molecular beacons with JOE dye: Influence of linker and 3' couple quencher.
Authors: Tsybulsky, Dmitry A and Kvach, Maksim V and Ryazantsev, Dmitry Y and Aparin, Ilya O and Stakheev, Alexander A and Prokhorenko, Igor A and Martynenko, Yury V and Gontarev, Sergey V and Formanovsky, Andrey A and Zatsepin, Timofei S and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K
Journal: Molecular and cellular probes (2016): 285-290
Authors: Tsybulsky, Dmitry A and Kvach, Maksim V and Ryazantsev, Dmitry Y and Aparin, Ilya O and Stakheev, Alexander A and Prokhorenko, Igor A and Martynenko, Yury V and Gontarev, Sergey V and Formanovsky, Andrey A and Zatsepin, Timofei S and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K
Journal: Molecular and cellular probes (2016): 285-290
Short PNA molecular beacons for real-time PCR allelic discrimination of single nucleotide polymorphisms.
Authors: Petersen, Kenneth and Vogel, Ulla and Rockenbauer, Eszter and Nielsen, Kirsten Vang and Kølvraa, Steen and Bolund, Lars and Nexø, Bjørn
Journal: Molecular and cellular probes (2004): 117-22
Authors: Petersen, Kenneth and Vogel, Ulla and Rockenbauer, Eszter and Nielsen, Kirsten Vang and Kølvraa, Steen and Bolund, Lars and Nexø, Bjørn
Journal: Molecular and cellular probes (2004): 117-22