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6-JOE azide

6-JOE is widely used in nucleic acid sequencing and related research. 6-JOE azide provides a convenient method for labeling alkyne-modified oligos throught the well-known click chemistry.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-JOE azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM174.417 µL872.083 µL1.744 mL8.721 mL17.442 mL
5 mM34.883 µL174.417 µL348.833 µL1.744 mL3.488 mL
10 mM17.442 µL87.208 µL174.417 µL872.083 µL1.744 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-FAM Azide493517830000.178
6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3*520545750001-
6-HEX azide533559730000.15
6-TET azide521542730000.13
6-TAMRA azide552578900000.178
6-ROX azide578604820000.168
6-JOE Phosphoramidite520545750001-
6-NED azide545567740001-
6-OG488 Azide498526760000.12
6-VIC Azide526543--
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References

View all 1 references: Citation Explorer
Detection and typing of Herpes Simplex virus (HSV) in mucocutaneous samples by TaqMan PCR targeting a gB segment homologous for HSV types 1 and 2
Authors: Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal: J Clin Microbiol (2005): 2058
Page updated on December 11, 2024

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Physical properties

Molecular weight

573.34

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

750001

Excitation (nm)

520

Emission (nm)

545

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Alternative formats