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AAT Bioquest

6-JOE azide

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight573.34
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)750001
Excitation (nm)520
Emission (nm)545
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Alternative formats
6-JOE alkyne

OverviewpdfSDSpdfProtocol


Molecular weight
573.34
Extinction coefficient (cm -1 M -1)
750001
Excitation (nm)
520
Emission (nm)
545
6-JOE is widely used in nucleic acid sequencing and related research. 6-JOE azide provides a convenient method for labeling alkyne-modified oligos throught the well-known click chemistry.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-JOE azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM174.417 µL872.083 µL1.744 mL8.721 mL17.442 mL
5 mM34.883 µL174.417 µL348.833 µL1.744 mL3.488 mL
10 mM17.442 µL87.208 µL174.417 µL872.083 µL1.744 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)750001
Excitation (nm)520
Emission (nm)545

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-FAM Azide493517830000.178
6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3*520545750001-
6-HEX azide533559730000.15
6-TET azide521542730000.13
6-TAMRA azide552578900000.178
6-ROX azide578604820000.168
6-JOE Phosphoramidite520545750001-
6-NED azide545567740001-
6-OG488 Azide498526760000.12

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References


View all 1 references: Citation Explorer
Detection and typing of Herpes Simplex virus (HSV) in mucocutaneous samples by TaqMan PCR targeting a gB segment homologous for HSV types 1 and 2
Authors: Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal: J Clin Microbiol (2005): 2058