6-JOE azide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
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Physical properties

Molecular weight	573.34
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	520
Emission (nm)	545

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

6-JOE acid

6-JOE alkyne

Overview SDS Protocol

Molecular weight

573.34

Extinction coefficient (cm ^-1 M ^-1)

75000¹

Excitation (nm)

520

Emission (nm)

545

6-JOE is widely used in nucleic acid sequencing and related research. 6-JOE azide provides a convenient method for labeling alkyne-modified oligos throught the well-known click chemistry.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-JOE azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	174.417 µL	872.083 µL	1.744 mL	8.721 mL	17.442 mL
5 mM	34.883 µL	174.417 µL	348.833 µL	1.744 mL	3.488 mL
10 mM	17.442 µL	87.208 µL	174.417 µL	872.083 µL	1.744 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	520
Emission (nm)	545

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-FAM Azide	493	517	83000	0.178
6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] CAS#: 113394-23-3	520	545	75000¹	-
6-HEX azide	533	559	73000	0.15
6-TET azide	521	542	73000	0.13
6-TAMRA azide	552	578	90000	0.178
6-ROX azide	578	604	82000	0.168
6-JOE Phosphoramidite	520	545	75000¹	-
6-NED azide	545	567	74000¹	-
6-OG488 Azide	498	526	76000	0.12
6-VIC Azide	526	543	-	-
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Images

Figure 1. Chemical structure for 6-JOE azide

References

View all 1 references: Citation Explorer

Detection and typing of Herpes Simplex virus (HSV) in mucocutaneous samples by TaqMan PCR targeting a gB segment homologous for HSV types 1 and 2
Authors: Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal: J Clin Microbiol (2005): 2058