Name: 6-JOE BCN
Brand: AAT Bioquest
SKU: 70523
Price: 115 USD
Availability: InStock

6-JOE BCN

6-JOE BCN is a bright, photostable yellow-green fluorescent dye used for site-specific labeling of oligonucleotides and other biomolecules via copper-free click chemistry.

Bioorthogonal: Selective for azides or tetrazines with minimal biological interference.
Fast kinetics: BCN reacts more rapidly than most other click chemistries.
Catalyst-free: The reaction does not require a toxic metal catalyst such as copper.
High biocompatibility: The reaction occurs under physiological pH and room temperature.
Versatility: It can be used for site-specific modification of a wide range of macromolecules.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
70523	1 mg	Price

Overview

6-JOE BCN is a clickable derivative of 6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein (6-JOE) widely used for labeling biomolecules such as oligonucleotides. 6-JOE is a popular yellow-green fluorescent dye known for its strong fluorescence, high photostability, and minimal pH sensitivity within the physiological range. To improve bioconjugation performance, it incorporates a PEG spacer that reduces steric hindrance and minimizes interference from the binding site of the resulting conjugate. The bicyclononyne (BCN) moiety enables strain-promoted azide–alkyne cycloaddition (SPAAC) with azido groups, forming stable triazole linkages under catalyst-free conditions. In addition, unlike dibenzocyclooctyne (DBCO), BCN also reacts efficiently with tetrazines through an inverse electron-demand Diels–Alder (IEDDA) reaction. This reaction is rapid, selective, and bioorthogonal, allowing labeling of biomolecules under physiological conditions without the need for metal catalysts or disruption of native biological processes.

Physical properties

Solvent

DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	520
Emission (nm)	545

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on May 9, 2026