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6-TAMRA C6 maleimide

6-TAMRA C6 maleimide is an excellent replacement for tetramethylrhodamine-5-maleimide. It selectively reacts with thiol groups of peptides, proteins and other biological molecules (such as thiol-modified oligonucleotides and small thiol-containing organic molecules). It might give higher conjugation yield than tetramethylrhodamine-6-maleimide under similar conjugation conditions. 6-TMR C6 maleimide has the spectral properties identical to those of tetramethylrhodamine-6-maleimide, thus all the instrument settings of the tetramethylrhodamine-5-maleimide would be applicable to the applications of 6-TMR C6 maleimide conjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TAMRA C6 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM160.346 µL801.732 µL1.603 mL8.017 mL16.035 mL
5 mM32.069 µL160.346 µL320.693 µL1.603 mL3.207 mL
10 mM16.035 µL80.173 µL160.346 µL801.732 µL1.603 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] *Mixed isomers*552578900000.320.178
5-TAMRA C6 maleimide552578900000.320.178

Citations

View all 2 citations: Citation Explorer
Voltage-dependent G-protein regulation of CaV2. 2 (N-type) channels
Authors: Nilsson, Michelle and Wang, Kaiqian and M{\'\i}nguez-Vi{\~n}as, Teresa and Angelini, Marina and Berglund, Stina and Olcese, Riccardo and Pantazis, Antonios
Journal: Science Advances (2024): eadp6665

References

View all 21 references: Citation Explorer
Severing of F-actin by yeast cofilin is pH-independent
Authors: Pavlov D, Muhlrad A, Cooper J, Wear M, Reisler E.
Journal: Cell Motil Cytoskeleton (2006): 533
Fluorometric measurements of intermolecular distances between the alpha and beta subunits of the Na+/K+-ATPase
Authors: Dempski RE, Hartung K, Friedrich T, Bamberg E.
Journal: J Biol Chem. (2006)
4-aminopyridine prevents the conformational changes associated with P/C-type inactivation in Shaker channels
Authors: Claydon TW, Vaid M, Rezazadeh S, Kehl SJ, Fedida D.
Journal: J Pharmacol Exp Ther. (2006)
Both rotor and stator subunits are necessary for efficient binding of F1 to F0 in functionally assembled Escherichia coli ATP synthase
Authors: Krebstakies T, Zimmermann B, Graber P, Altendorf K, Borsch M, Greie JC.
Journal: J Biol Chem (2005): 33338
The beta subunit of the Na+/K+-ATPase follows the conformational state of the holoenzyme
Authors: Dempski RE, Friedrich T, Bamberg E.
Journal: J Gen Physiol (2005): 505
Page updated on October 12, 2024

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Physical properties

Molecular weight

623.65

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm -1 M -1)

90000

Excitation (nm)

552

Emission (nm)

578

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501