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7-Hydroxycoumarin-3-carboxylic acid *CAS 779-27-1*

7-Hydroxycoumarin-3-carboxylic acid is a blue fluorophores for labeling proteins and nucleic acids mostly through the in situ formation of its succinimidyl ester catalyzed by EDAC.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxycoumarin-3-carboxylic acid *CAS 779-27-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM485.084 µL2.425 mL4.851 mL24.254 mL48.508 mL
5 mM97.017 µL485.084 µL970.167 µL4.851 mL9.702 mL
10 mM48.508 µL242.542 µL485.084 µL2.425 mL4.851 mL

Molarity calculator

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Correction Factor (280 nm)
7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8*3554050.171

References

View all 16 references: Citation Explorer
In vitro anti-tumour and cyto-selective effects of coumarin-3-carboxylic acid and three of its hydroxylated derivatives, along with their silver-based complexes, using human epithelial carcinoma cell lines
Authors: Thati B, Noble A, Creaven BS, Walsh M, McCann M, Kavanagh K, Devereux M, Egan DA.
Journal: Cancer Lett. (2006)
Effects of iron on Vitamin C/copper-induced hydroxyl radical generation in bicarbonate-rich water
Authors: Jansson PJ, Del Castillo U, Lindqvist C, Nordstrom T.
Journal: Free Radic Res (2005): 565
Induction of 4-hydroxycinnamate decarboxylase in Klebsiella oxytoca cells exposed to substrates and non-substrate 4-hydroxycinnamate analogs
Authors: Hashidoko Y, Tanaka T, Tahara S.
Journal: Biosci Biotechnol Biochem (2001): 2604
A fluorometric assay for glycosyltransferase activities using sugars aminated and tagged with 7-hydroxycoumarin-3-carboxylic acid as substrates and high performance liquid chromatography
Authors: Higai K, Masuda D, Matsuzawa Y, Satoh T, Matsumoto K.
Journal: Biol Pharm Bull (1999): 333
Flow injection analysis of binding reaction between fluorescent lectin and cells
Authors: Oda Y, Kinoshita M, Nakayama K, Ikeda S, Kakehi K.
Journal: Anal Biochem (1999): 230
Page updated on October 4, 2024

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Physical properties

Molecular weight

206.15

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.0815

Excitation (nm)

352

Emission (nm)

407

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

779-27-1
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