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7-Hydroxycoumarin-3-carboxylic acid *CAS 779-27-1*

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Physical properties
Molecular weight206.15
Spectral properties
Correction Factor (280 nm)0.0815
Excitation (nm)352
Emission (nm)407
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Coumarins
Molecular weight
Correction Factor (280 nm)
Excitation (nm)
Emission (nm)
7-Hydroxycoumarin-3-carboxylic acid is a blue fluorophores for labeling proteins and nucleic acids mostly through the in situ formation of its succinimidyl ester catalyzed by EDAC.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxycoumarin-3-carboxylic acid *CAS 779-27-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM485.084 µL2.425 mL4.851 mL24.254 mL48.508 mL
5 mM97.017 µL485.084 µL970.167 µL4.851 mL9.702 mL
10 mM48.508 µL242.542 µL485.084 µL2.425 mL4.851 mL

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Spectral properties

Correction Factor (280 nm)0.0815
Excitation (nm)352
Emission (nm)407

Product Family

NameExcitation (nm)Emission (nm)Correction Factor (280 nm)
7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8*3554050.171



View all 16 references: Citation Explorer
In vitro anti-tumour and cyto-selective effects of coumarin-3-carboxylic acid and three of its hydroxylated derivatives, along with their silver-based complexes, using human epithelial carcinoma cell lines
Authors: Thati B, Noble A, Creaven BS, Walsh M, McCann M, Kavanagh K, Devereux M, Egan DA.
Journal: Cancer Lett. (2006)
Effects of iron on Vitamin C/copper-induced hydroxyl radical generation in bicarbonate-rich water
Authors: Jansson PJ, Del Castillo U, Lindqvist C, Nordstrom T.
Journal: Free Radic Res (2005): 565
Induction of 4-hydroxycinnamate decarboxylase in Klebsiella oxytoca cells exposed to substrates and non-substrate 4-hydroxycinnamate analogs
Authors: Hashidoko Y, Tanaka T, Tahara S.
Journal: Biosci Biotechnol Biochem (2001): 2604
Fluorescent labelling of closely-spaced aldehydes induced in DNA by bleomycin-Fe(III)
Authors: Chakrabarti S, Mahmood A, Makrigiorgos GM.
Journal: Int J Radiat Biol (1999): 1055
Flow injection analysis of binding reaction between fluorescent lectin and cells
Authors: Oda Y, Kinoshita M, Nakayama K, Ikeda S, Kakehi K.
Journal: Anal Biochem (1999): 230
A fluorometric assay for glycosyltransferase activities using sugars aminated and tagged with 7-hydroxycoumarin-3-carboxylic acid as substrates and high performance liquid chromatography
Authors: Higai K, Masuda D, Matsuzawa Y, Satoh T, Matsumoto K.
Journal: Biol Pharm Bull (1999): 333
Autoxidation of ferrous ion complexes: a method for the generation of hydroxyl radicals
Authors: Kachur AV, Tuttle SW, Biaglow JE.
Journal: Radiat Res (1998): 475
Trans/cis (Z/E) photoisomerization of the chromophore of photoactive yellow protein is not a prerequisite for the initiation of the photocycle of this photoreceptor protein
Authors: Cordfunke R, Kort R, Pierik A, Gobets B, Koomen GJ, Verhoeven JW, Hellingwerf KJ.
Journal: Proc Natl Acad Sci U S A (1998): 7396
Coumarin-3-carboxylic acid as a detector for hydroxyl radicals generated chemically and by gamma radiation
Authors: Manevich Y, Held KD, Biaglow JE.
Journal: Radiat Res (1997): 580
Effect of purine nucleoside phosphates on OH-radical generation by reaction of Fe2+ with oxygen
Authors: Kachur AV, Manevich Y, Biaglow JE.
Journal: Free Radic Res (1997): 399