7-Hydroxycoumarin-3-carboxylic acid, succinimidyl ester CAS 134471-24-2

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Physical properties

Molecular weight	303.23
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.0815
Excitation (nm)	352
Emission (nm)	407

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

134471-24-2

Molecular weight

303.23

Correction Factor (280 nm)

0.0815

Excitation (nm)

352

Emission (nm)

407

7-Hydroxycoumarin-3-carboxylic acid, SE is the amine-reactive succinimidyl ester of 7-Hydroxycoumarin-3-carboxylic acid. It is a blue fluorescent dyes for labeling proteins and nucleic acids. This coumarin is also increasingly used to label peptides, nucleotides and carbohydrates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 134471-24-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	329.783 µL	1.649 mL	3.298 mL	16.489 mL	32.978 mL
5 mM	65.957 µL	329.783 µL	659.565 µL	3.298 mL	6.596 mL
10 mM	32.978 µL	164.891 µL	329.783 µL	1.649 mL	3.298 mL

Molarity calculator

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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.0815
Excitation (nm)	352
Emission (nm)	407

Product Family

Name	Excitation (nm)	Emission (nm)	Correction Factor (280 nm)
7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester CAS 150321-92-9	355	405	0.171

Images

Figure 1. Chemical structure for 7-Hydroxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 134471-24-2*

References

View all 16 references: Citation Explorer

In vitro anti-tumour and cyto-selective effects of coumarin-3-carboxylic acid and three of its hydroxylated derivatives, along with their silver-based complexes, using human epithelial carcinoma cell lines
Authors: Thati B, Noble A, Creaven BS, Walsh M, McCann M, Kavanagh K, Devereux M, Egan DA.
Journal: Cancer Lett. (2006)

Effects of iron on Vitamin C/copper-induced hydroxyl radical generation in bicarbonate-rich water
Authors: Jansson PJ, Del Castillo U, Lindqvist C, Nordstrom T.
Journal: Free Radic Res (2005): 565

Induction of 4-hydroxycinnamate decarboxylase in Klebsiella oxytoca cells exposed to substrates and non-substrate 4-hydroxycinnamate analogs
Authors: Hashidoko Y, Tanaka T, Tahara S.
Journal: Biosci Biotechnol Biochem (2001): 2604

Fluorescent labelling of closely-spaced aldehydes induced in DNA by bleomycin-Fe(III)
Authors: Chakrabarti S, Mahmood A, Makrigiorgos GM.
Journal: Int J Radiat Biol (1999): 1055

Flow injection analysis of binding reaction between fluorescent lectin and cells
Authors: Oda Y, Kinoshita M, Nakayama K, Ikeda S, Kakehi K.
Journal: Anal Biochem (1999): 230

A fluorometric assay for glycosyltransferase activities using sugars aminated and tagged with 7-hydroxycoumarin-3-carboxylic acid as substrates and high performance liquid chromatography
Authors: Higai K, Masuda D, Matsuzawa Y, Satoh T, Matsumoto K.
Journal: Biol Pharm Bull (1999): 333

Autoxidation of ferrous ion complexes: a method for the generation of hydroxyl radicals
Authors: Kachur AV, Tuttle SW, Biaglow JE.
Journal: Radiat Res (1998): 475

Trans/cis (Z/E) photoisomerization of the chromophore of photoactive yellow protein is not a prerequisite for the initiation of the photocycle of this photoreceptor protein
Authors: Cordfunke R, Kort R, Pierik A, Gobets B, Koomen GJ, Verhoeven JW, Hellingwerf KJ.
Journal: Proc Natl Acad Sci U S A (1998): 7396

Coumarin-3-carboxylic acid as a detector for hydroxyl radicals generated chemically and by gamma radiation
Authors: Manevich Y, Held KD, Biaglow JE.
Journal: Radiat Res (1997): 580

Effect of purine nucleoside phosphates on OH-radical generation by reaction of Fe2+ with oxygen
Authors: Kachur AV, Manevich Y, Biaglow JE.
Journal: Free Radic Res (1997): 399