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7-Hydroxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 134471-24-2*

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Physical properties
Molecular weight303.23
Spectral properties
Correction Factor (280 nm)0.0815
Excitation (nm)352
Emission (nm)407
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (280 nm)
Excitation (nm)
Emission (nm)
7-Hydroxycoumarin-3-carboxylic acid, SE is the amine-reactive succinimidyl ester of 7-Hydroxycoumarin-3-carboxylic acid. It is a blue fluorescent dyes for labeling proteins and nucleic acids. This coumarin is also increasingly used to label peptides, nucleotides and carbohydrates.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 134471-24-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM329.783 µL1.649 mL3.298 mL16.489 mL32.978 mL
5 mM65.957 µL329.783 µL659.565 µL3.298 mL6.596 mL
10 mM32.978 µL164.891 µL329.783 µL1.649 mL3.298 mL

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Spectral properties

Correction Factor (280 nm)0.0815
Excitation (nm)352
Emission (nm)407

Product Family

NameExcitation (nm)Emission (nm)Correction Factor (280 nm)
7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 150321-92-9*3554050.171



View all 16 references: Citation Explorer
In vitro anti-tumour and cyto-selective effects of coumarin-3-carboxylic acid and three of its hydroxylated derivatives, along with their silver-based complexes, using human epithelial carcinoma cell lines
Authors: Thati B, Noble A, Creaven BS, Walsh M, McCann M, Kavanagh K, Devereux M, Egan DA.
Journal: Cancer Lett. (2006)
Effects of iron on Vitamin C/copper-induced hydroxyl radical generation in bicarbonate-rich water
Authors: Jansson PJ, Del Castillo U, Lindqvist C, Nordstrom T.
Journal: Free Radic Res (2005): 565
Induction of 4-hydroxycinnamate decarboxylase in Klebsiella oxytoca cells exposed to substrates and non-substrate 4-hydroxycinnamate analogs
Authors: Hashidoko Y, Tanaka T, Tahara S.
Journal: Biosci Biotechnol Biochem (2001): 2604
Fluorescent labelling of closely-spaced aldehydes induced in DNA by bleomycin-Fe(III)
Authors: Chakrabarti S, Mahmood A, Makrigiorgos GM.
Journal: Int J Radiat Biol (1999): 1055
Flow injection analysis of binding reaction between fluorescent lectin and cells
Authors: Oda Y, Kinoshita M, Nakayama K, Ikeda S, Kakehi K.
Journal: Anal Biochem (1999): 230
A fluorometric assay for glycosyltransferase activities using sugars aminated and tagged with 7-hydroxycoumarin-3-carboxylic acid as substrates and high performance liquid chromatography
Authors: Higai K, Masuda D, Matsuzawa Y, Satoh T, Matsumoto K.
Journal: Biol Pharm Bull (1999): 333
Autoxidation of ferrous ion complexes: a method for the generation of hydroxyl radicals
Authors: Kachur AV, Tuttle SW, Biaglow JE.
Journal: Radiat Res (1998): 475
Trans/cis (Z/E) photoisomerization of the chromophore of photoactive yellow protein is not a prerequisite for the initiation of the photocycle of this photoreceptor protein
Authors: Cordfunke R, Kort R, Pierik A, Gobets B, Koomen GJ, Verhoeven JW, Hellingwerf KJ.
Journal: Proc Natl Acad Sci U S A (1998): 7396
Coumarin-3-carboxylic acid as a detector for hydroxyl radicals generated chemically and by gamma radiation
Authors: Manevich Y, Held KD, Biaglow JE.
Journal: Radiat Res (1997): 580
Effect of purine nucleoside phosphates on OH-radical generation by reaction of Fe2+ with oxygen
Authors: Kachur AV, Manevich Y, Biaglow JE.
Journal: Free Radic Res (1997): 399