7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8*
7-Methoxycoumarin-3-carboxylic acid is used for HPLC derivatization. It is also used to develop FRET probes for analyzing protease activities.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 454.174 µL | 2.271 mL | 4.542 mL | 22.709 mL | 45.417 mL |
5 mM | 90.835 µL | 454.174 µL | 908.348 µL | 4.542 mL | 9.083 mL |
10 mM | 45.417 µL | 227.087 µL | 454.174 µL | 2.271 mL | 4.542 mL |
Molarity calculator
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Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Correction Factor (280 nm) |
7-Hydroxycoumarin-3-carboxylic acid *CAS 779-27-1* | 352 | 407 | 0.0815 |
References
View all 3 references: Citation Explorer
Synthesis of N epsilon-(7-diethylaminocoumarin-3-carboxyl)- and N epsilon-(7-methoxycoumarin-3-carboxyl)-L-Fmoc lysine as tools for protease cleavage detection by fluorescence
Authors: Berthelot T, Talbot JC, Lain G, Deleris G, Latxague L.
Journal: J Pept Sci (2005): 153
Authors: Berthelot T, Talbot JC, Lain G, Deleris G, Latxague L.
Journal: J Pept Sci (2005): 153
An alternative quenched fluorescence substrate for Pz-peptidase
Authors: Tisljar U, et al.
Journal: Anal Biochem (1990): 112-5
Authors: Tisljar U, et al.
Journal: Anal Biochem (1990): 112-5
Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131
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