8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt [HPTS] CAS 6358-69-6

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Physical properties

Molecular weight	524.38
Solvent	Water

Spectral properties

Excitation (nm)	452
Emission (nm)	512

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

6358-69-6

Molecular weight

524.38

Excitation (nm)

452

Emission (nm)

512

8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt [HPTS] is the cell-impermeable fluorescent pH indicator used for slightly acidic pH range (pKa = 6.4). It is highly water-soluble.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of 8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt [HPTS] *CAS 6358-69-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	190.701 µL	953.507 µL	1.907 mL	9.535 mL	19.07 mL
5 mM	38.14 µL	190.701 µL	381.403 µL	1.907 mL	3.814 mL
10 mM	19.07 µL	95.351 µL	190.701 µL	953.507 µL	1.907 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	452
Emission (nm)	512

Images

Figure 1. Chemical structure for 8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt [HPTS] *CAS 6358-69-6*

Citations

View all 1 citations: Citation Explorer

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Authors: Dobson, Chandler and Zielke, Claudia and Pan, Ching W and Feit, Cameron and Abbyad, Paul
Journal: Micromachines (2020): 964

References

View all 20 references: Citation Explorer

Temperature dependence of excited-state proton transfer in water electrolyte solutions and water-methanol solutions
Authors: Leiderman P, Gepshtein R, Uritski A, Genosar L, Huppert D.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 9039

Emission-based optical carbon dioxide sensing with HPTS in green chemistry reagents: room-temperature ionic liquids
Authors: Oter O, Ertekin K, Topkaya D, Alp S.
Journal: Anal Bioanal Chem (2006): 1225

The effect of neuronal morphology and membrane-permeant weak acid and base on the dissipation of depolarization-induced pH gradients in snail neurons
Authors: Pantazis A, Keegan P, Postma M, Schwiening CJ.
Journal: Pflugers Arch (2006): 175

Effect of electrolytes on the excited-state proton transfer and geminate recombination
Authors: Leiderman P, Gepshtein R, Uritski A, Genosar L, Huppert D.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 5573

Universal behaviour of gel formation from acrylamide-carrageenan mixture around the gel point: a fluorescence study
Authors: Aktas DK, Evingur GA, Pekcan O.
Journal: J Biomol Struct Dyn (2006): 83

Testing the three step excited state proton transfer model by the effect of an excess proton
Authors: Gepshtein R, Leiderman P, Genosar L, Huppert D.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 9674

Indirect laser-induced fluorescence detection for capillary electrophoresis using a frequency-doubled diode laser
Authors: Ragozina N, Putz M, Faubel W, Pyell U.
Journal: Electrophoresis (2003): 567

High-stability non-invasive autoclavable naked optical CO2 sensor
Authors: Ge X, Kostov Y, Rao G.
Journal: Biosens Bioelectron (2003): 857

Depolarization-induced pH microdomains and their relationship to calcium transients in isolated snail neurones
Authors: Schwiening CJ, Willoughby D.
Journal: J Physiol (2002): 371

The effects of intracellular pH changes on resting cytosolic calcium in voltage-clamped snail neurones
Authors: Willoughby D, Thomas R, Schwiening C.
Journal: J Physiol (2001): 405