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8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt [HPTS] *CAS 6358-69-6*

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InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight524.38
Spectral properties
Excitation (nm)452
Emission (nm)512
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt [HPTS] is the cell-impermeable fluorescent pH indicator used for slightly acidic pH range (pKa = 6.4). It is highly water-soluble.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of 8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt [HPTS] *CAS 6358-69-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM190.701 µL953.507 µL1.907 mL9.535 mL19.07 mL
5 mM38.14 µL190.701 µL381.403 µL1.907 mL3.814 mL
10 mM19.07 µL95.351 µL190.701 µL953.507 µL1.907 mL

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Spectral properties

Excitation (nm)452
Emission (nm)512



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View all 20 references: Citation Explorer
Temperature dependence of excited-state proton transfer in water electrolyte solutions and water-methanol solutions
Authors: Leiderman P, Gepshtein R, Uritski A, Genosar L, Huppert D.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 9039
Emission-based optical carbon dioxide sensing with HPTS in green chemistry reagents: room-temperature ionic liquids
Authors: Oter O, Ertekin K, Topkaya D, Alp S.
Journal: Anal Bioanal Chem (2006): 1225
The effect of neuronal morphology and membrane-permeant weak acid and base on the dissipation of depolarization-induced pH gradients in snail neurons
Authors: Pantazis A, Keegan P, Postma M, Schwiening CJ.
Journal: Pflugers Arch (2006): 175
Effect of electrolytes on the excited-state proton transfer and geminate recombination
Authors: Leiderman P, Gepshtein R, Uritski A, Genosar L, Huppert D.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 5573
Universal behaviour of gel formation from acrylamide-carrageenan mixture around the gel point: a fluorescence study
Authors: Aktas DK, Evingur GA, Pekcan O.
Journal: J Biomol Struct Dyn (2006): 83
Testing the three step excited state proton transfer model by the effect of an excess proton
Authors: Gepshtein R, Leiderman P, Genosar L, Huppert D.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 9674
Indirect laser-induced fluorescence detection for capillary electrophoresis using a frequency-doubled diode laser
Authors: Ragozina N, Putz M, Faubel W, Pyell U.
Journal: Electrophoresis (2003): 567
High-stability non-invasive autoclavable naked optical CO2 sensor
Authors: Ge X, Kostov Y, Rao G.
Journal: Biosens Bioelectron (2003): 857
Depolarization-induced pH microdomains and their relationship to calcium transients in isolated snail neurones
Authors: Schwiening CJ, Willoughby D.
Journal: J Physiol (2002): 371
The effects of intracellular pH changes on resting cytosolic calcium in voltage-clamped snail neurones
Authors: Willoughby D, Thomas R, Schwiening C.
Journal: J Physiol (2001): 405