8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt [HPTS] *CAS 6358-69-6*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 524.38 |
Solvent | Water |
Spectral properties
Excitation (nm) | 452 |
Emission (nm) | 512 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Intracellular pH, Physiological Probes
CAS 6358-69-6 | Molecular weight 524.38 | Excitation (nm) 452 | Emission (nm) 512 |
8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt [HPTS] is the cell-impermeable fluorescent pH indicator used for slightly acidic pH range (pKa = 6.4). It is highly water-soluble.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of 8-Hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt [HPTS] *CAS 6358-69-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 190.701 µL | 953.507 µL | 1.907 mL | 9.535 mL | 19.07 mL |
5 mM | 38.14 µL | 190.701 µL | 381.403 µL | 1.907 mL | 3.814 mL |
10 mM | 19.07 µL | 95.351 µL | 190.701 µL | 953.507 µL | 1.907 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Citations
View all 1 citations: Citation Explorer
Method for Passive Droplet Sorting after Photo-Tagging
Authors: Dobson, Chandler and Zielke, Claudia and Pan, Ching W and Feit, Cameron and Abbyad, Paul
Journal: Micromachines (2020): 964
Authors: Dobson, Chandler and Zielke, Claudia and Pan, Ching W and Feit, Cameron and Abbyad, Paul
Journal: Micromachines (2020): 964
References
View all 20 references: Citation Explorer
Temperature dependence of excited-state proton transfer in water electrolyte solutions and water-methanol solutions
Authors: Leiderman P, Gepshtein R, Uritski A, Genosar L, Huppert D.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 9039
Authors: Leiderman P, Gepshtein R, Uritski A, Genosar L, Huppert D.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 9039
Emission-based optical carbon dioxide sensing with HPTS in green chemistry reagents: room-temperature ionic liquids
Authors: Oter O, Ertekin K, Topkaya D, Alp S.
Journal: Anal Bioanal Chem (2006): 1225
Authors: Oter O, Ertekin K, Topkaya D, Alp S.
Journal: Anal Bioanal Chem (2006): 1225
The effect of neuronal morphology and membrane-permeant weak acid and base on the dissipation of depolarization-induced pH gradients in snail neurons
Authors: Pantazis A, Keegan P, Postma M, Schwiening CJ.
Journal: Pflugers Arch (2006): 175
Authors: Pantazis A, Keegan P, Postma M, Schwiening CJ.
Journal: Pflugers Arch (2006): 175
Effect of electrolytes on the excited-state proton transfer and geminate recombination
Authors: Leiderman P, Gepshtein R, Uritski A, Genosar L, Huppert D.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 5573
Authors: Leiderman P, Gepshtein R, Uritski A, Genosar L, Huppert D.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 5573
Universal behaviour of gel formation from acrylamide-carrageenan mixture around the gel point: a fluorescence study
Authors: Aktas DK, Evingur GA, Pekcan O.
Journal: J Biomol Struct Dyn (2006): 83
Authors: Aktas DK, Evingur GA, Pekcan O.
Journal: J Biomol Struct Dyn (2006): 83
Testing the three step excited state proton transfer model by the effect of an excess proton
Authors: Gepshtein R, Leiderman P, Genosar L, Huppert D.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 9674
Authors: Gepshtein R, Leiderman P, Genosar L, Huppert D.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 9674
Indirect laser-induced fluorescence detection for capillary electrophoresis using a frequency-doubled diode laser
Authors: Ragozina N, Putz M, Faubel W, Pyell U.
Journal: Electrophoresis (2003): 567
Authors: Ragozina N, Putz M, Faubel W, Pyell U.
Journal: Electrophoresis (2003): 567
High-stability non-invasive autoclavable naked optical CO2 sensor
Authors: Ge X, Kostov Y, Rao G.
Journal: Biosens Bioelectron (2003): 857
Authors: Ge X, Kostov Y, Rao G.
Journal: Biosens Bioelectron (2003): 857
Depolarization-induced pH microdomains and their relationship to calcium transients in isolated snail neurones
Authors: Schwiening CJ, Willoughby D.
Journal: J Physiol (2002): 371
Authors: Schwiening CJ, Willoughby D.
Journal: J Physiol (2002): 371
The effects of intracellular pH changes on resting cytosolic calcium in voltage-clamped snail neurones
Authors: Willoughby D, Thomas R, Schwiening C.
Journal: J Physiol (2001): 405
Authors: Willoughby D, Thomas R, Schwiening C.
Journal: J Physiol (2001): 405
Application notes
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
FAQ
How to measure intracellular pH using flow cytometry?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What is an AM ester?
Are NADH and ROS related?
Are there any alternatives for ethidium bromide in agarose gels?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What is an AM ester?
Are NADH and ROS related?
Are there any alternatives for ethidium bromide in agarose gels?