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AMCA Ethylenediamine

AMCA is one of the brightest blue fluorescent labeling dyes. It can be well excited at 365 nm. It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. This AMC dye can be used for labeling carbonyl compounds (such as carboxy compounds).
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Catalog Number504
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Physical properties
Molecular weight503.35
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.153
Extinction coefficient (cm -1 M -1)19000
Excitation (nm)346
Emission (nm)434
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AMCA Ethylenediamine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM198.669 µL993.345 µL1.987 mL9.933 mL19.867 mL
5 mM39.734 µL198.669 µL397.338 µL1.987 mL3.973 mL
10 mM19.867 µL99.334 µL198.669 µL993.345 µL1.987 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum
Product family
NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
AMCA acid *CAS#: 106562-32-7*346434190000.153
AMCA Alkyne346434190000.153
AMCA Azide346434190000.153
References
View all 13 references: Citation Explorer
Functional expression of the peptide transporter PEPT2 in the mammalian enteric nervous system
Authors: Ruhl A, Hoppe S, Frey I, Daniel H, Schemann M.
Journal: J Comp Neurol (2005): 1
Increased resonance energy transfer between fluorophores bound to DNA in proximity to metallic silver particles
Authors: Malicka J, Gryczynski I, Fang J, Kusba J, Lakowicz JR.
Journal: Anal Biochem (2003): 160
Dental extractions in patients maintained on oral anticoagulant therapy: comparison of INR value with occurrence of postoperative bleeding
Authors: Blinder D, Manor Y, Martinowitz U, Taicher S.
Journal: Int J Oral Maxillofac Surg (2001): 518
Hemostatic wound management in marcumar patients. Collagen fleece vs. tranexamic acid
Authors: Bublitz R, Sommer S, Weingart D, Bauerle K, Both A.
Journal: Mund Kiefer Gesichtschir (2000): 240
Oral surgical procedures during anticoagulant therapy
Authors: Blinder D, Martinowitz U, Ardekian L, Peleg M, Taicher S.
Journal: Harefuah (1996): 681