Amplite® Colorimetric Beta-Galactosidase Assay Kit

Amplite® Colorimetric Beta-Galactosidase Assay Kit is a colorimetric assay kit for detecting and quantifying beta-galactosidase activity in cells and assay systems.

Colorimetric detection: Uses a chromogenic galactosidase substrate that generates a purple product upon enzyme reaction.
High sensitivity: Detects beta-galactosidase activity as low as 3 mU/mL after 30-60 minutes.
Galactosidase substrate: Uses Resorufin β-D-Galactoside for enzyme activity detection.
Microplate compatible: Read absorbance at 580/460 nm using a clear-bottom plate.
Applications: Suitable for LacZ expression, ELISA-type assays, and inhibitor or inducer screening.

&beta; -galactosidase dose response was measured with Amplite&trade; Colorimetric &beta;-Galactosidase Assay Kit (Cat #: 12604) in a 96-well clear bottom plate using a SpectraMax microplate reader (Molecular Devices). As low as 3 mU/mL &beta;- galactosidase was detected with 30-60 minutes incubation. (Note: The absorbance background increases with time, thus it is important to subtract the absorbance of the blank wells for each data point.)

Protocol

SDS

Catalog	Size	Price	Quantity
12604	200 Tests	Price

Overview

E. coli beta-galactosidase is a 464 kD tetramer. Each unit of beta-galactosidase consists of five domains, the third of which is the active site. It is an essential enzyme in cells. Deficiencies of this enzyme can result in galactosialidosis or Morquio B syndrome. In E. coli, beta-galactosidase is produced by the activation of LacZ operon. Detection of LacZ expression has become routine to the point of detection of as few as 5 copies of beta-galactosidase per cell. This kit uses a chromogenic galactosidase substrate that can sensitively distinguish LacZ+ from LacZ- cells. The light yellow substrate generates a strongly purple product upon reaction with galactosidase. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. Amplite® Colorimetric Beta-Galactosidase Assay Kit comes with all the essential components with an optimized assay protocol. It can be used for screening galactosidase inhibitors or inducers.

References

View all 23 references: Citation Explorer

Microfluidic sequential injection analysis in a short capillary

Authors:

Du WB, Fang Q, Fang ZL.

Journal:

Anal Chem (2006): 6404

Cellular logic with orthogonal ribosomes

Authors:

Rackham O, Chin JW.

Journal:

J Am Chem Soc (2005): 17584

New transport peptides broaden the horizon of applications for peptidic pharmaceuticals

Authors:

Langedijk JP, Olijhoek T, Schut D, Autar R, Meloen RH.

Journal:

Mol Divers (2004): 101

Integration of on-column immobilized enzyme reactor in microchip electrophoresis

Authors:

Park SS, Cho SI, Kim MS, Kim YK, Kim BG.

Journal:

Electrophoresis (2003): 200

Model for focal demyelination of the spinal dorsal columns of transgenic MBP-LacZ mice by phototargeted ablation of oligodendrocytes

Authors:

V, undefined and erluit JL, Bourque JA, Peterson AC, Tetzlaff W.

Journal:

J Neurosci Res (2000): 28

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	580/460 nm
Recommended plate	Clear bottom

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Page updated on October 8, 2026

Component A: Resorufin β-D-Galactoside	1 vial
Component B: Reaction Buffer	1 bottle (20 mL)
Component C: Stop Buffer	1 vial (10 mL)
Component D: Lysis Buffer	1 vial (10 mL)
Component E: DMSO	1 vial (100 µL)
Component F: β-Mercaptoethanol	1 vial (100 µL)