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Amplite® Colorimetric Beta-Galactosidase Assay Kit
E. coli beta-galactosidase is a 464 kD tetramer. Each unit of beta-galactosidase consists of five domains, the third of which is the active site. It is an essential enzyme in cells. Deficiencies of this enzyme can result in galactosialidosis or Morquio B syndrome. In E. coli, beta-galactosidase is produced by the activation of LacZ operon. Detection of LacZ expression has become routine to the point of detection of as few as 5 copies of beta-galactosidase per cell. This kit uses a chromogenic galactosidase substrate that can sensitively distinguish LacZ+ from LacZ- cells. The light yellow substrate generates a strongly purple product upon reaction with galactosidase. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. Amplite® Colorimetric Beta-Galactosidase Assay Kit comes with all the essential components with an optimized assay protocol. It can be used for screening galactosidase inhibitors or inducers.
β -galactosidase dose response was measured with Amplite™ Colorimetric β-Galactosidase Assay Kit (Cat #: 12604) in a 96-well clear bottom plate using a SpectraMax microplate reader (Molecular Devices). As low as 3 mU/mL β- galactosidase was detected with 30-60 minutes incubation. (Note: The absorbance background increases with time, thus it is important to subtract the absorbance of the blank wells for each data point.)
β -galactosidase dose response was measured with Amplite™ Colorimetric β-Galactosidase Assay Kit (Cat #: 12604) in a 96-well clear bottom plate using a SpectraMax microplate reader (Molecular Devices). As low as 3 mU/mL β- galactosidase was detected with 30-60 minutes incubation. (Note: The absorbance background increases with time, thus it is important to subtract the absorbance of the blank wells for each data point.)
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
12604200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Absorbance microplate reader
Absorbance580/460 nm
Recommended plateClear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Enzyme Probes & Assay Kits
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Page updated on October 29, 2025