Amplite® Colorimetric Superoxide Dismutase (SOD) Assay Kit *Enhanced Sensitivity*

Amplite® Colorimetric Superoxide Dismutase (SOD) Assay Kit

Enhanced Sensitivity

Amplite® Colorimetric Superoxide Dismutase (SOD) Assay Kit Enhanced Sensitivity is a colorimetric assay kit for quantifying SOD activity in a microplate format.

Detection Principle: Measures SOD-mediated inhibition of WST-1 reduction.
Signal Advantage: Produces a yellow formazan signal proportional to SOD activity.
Reaction Chemistry: Uses an NADH-based system to generate superoxide radicals.
Instrument Compatibility: Absorbance detection at 440 nm using a microplate reader.
Plate Format: Compatible with 96- and 384-well microplates.

OD dose response was measured with Amplite® Colorimetric Superoxide Dismutase Assay Kit in a 96-well white wall/clear bottom plate with a Spectrum Max microplate reader (Molecular Devices).

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
11308	200 Tests	Price

Overview

Superoxide dismutases (SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. Superoxide is one of the main reactive oxygen species in cells. It is a substantial contributor of pathology associated with neurodegenerative diseases, ischemia reperfusion injury, atherosclerosis and aging. SODs are an important antioxidant defense in nearly all cells exposed to superoxide radicals. In fact, mice lacking SOD1 develop a wide range of pathologies, including hepatocellular carcinoma, an acceleration of age-related muscle mass loss, an earlier incidence of cataracts and a reduced lifespan. Overexpression of SOD protects murine fibrosarcoma cells from apoptosis and promotes cell differentiation. Amplite® Colorimetric Superoxide Dismutase (SOD) Assay Kit provides a rapid and sensitive method for the measurement of SOD activity. It is well-known that NADH and SOD enzyme system generates superoxide radicals that reduce WST-1 into a yellow color formazan dye that has maximum absorption around 440 nm. SOD inhibits the reduction of WST-1 by catalyzing the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, thus reduces the 440 nm absorption of the formazan product. The reduction of 440 nm absorption is proportional to SOD activity. The kit can be performed in a convenient 96-well or 384-well microtiter-plate format.

Citations

View all 34 citations: Citation Explorer

Erinacine A-Enriched Hericium erinaceus Mycelium Ethanol Extract Lessens Cellular Damage in Cell and Drosophila Models of Spinocerebellar Ataxia Type 3 by Improvement of Nrf2 Activation

Authors:

Wu, Yu-Ling and Sun, Hai-Lun and Chang, Jui-Chih and Lin, Wei-Yong and Chen, Pei-Yin and Chen, Chin-Chu and Lee, Li-Ya and Li, Chien-Chun and Hsieh, Mingli and Chen, Haw-Wen and others,

Journal:

Antioxidants (2024): 1495

Intracellular pyruvate as one of the major bioactive substances of lactic acid bacteria isolated from kimchi

Authors:

Kang, Jin Yong and Lee, Moeun and Song, Jung Hee and Choi, Eun Ji and Mun, So Yeong and Kim, Daun and Lim, Seul Ki and Kim, Namhee and Park, Bo Yeon and Chang, Ji Yoon

Journal:

Journal of Food Science (2024)

Amelioration of AlCl3-induced Memory Loss in the Rats by an Aqueous Extract of Guduchi, a Medhya Rasayana

Authors:

Jamadagni, Shrirang B and Ghadge, Pooja M and Tambe, Mukul S and Srinivasan, Marimuthu and Prasad, Goli Penchala and Jamadagni, Pallavi S and Prasad, Shyam Baboo and Pawar, Sharad D and Gurav, Arun M and Gaidhani, Sudesh N and others,

Journal:

Pharmacognosy Magazine (2023): 09731296221145063

Accelerated sarcopenia in Cu/Zn superoxide dismutase knockout mice

Authors:

Deepa, S. S., Van Remmen, H., Brooks, S. V., Faulkner, J. A., Larkin, L., McArdle, A., Jackson, M. J., Vasilaki, A., Richardson, A.

Journal:

Free Radic Biol Med (2019): 19-23

The copper-zinc superoxide dismutase activity in selected diseases

Authors:

Lew, undefined and owski, L., Kepinska, M., Milnerowicz, H.

Journal:

Eur J Clin Invest (2019): e13036

References

View all 111 references: Citation Explorer

Association of cigarette smoking with superoxide dismutase enzyme levels in subjects with chronic periodontitis

Authors:

Agnihotri R, P and urang P, Kamath SU, Goyal R, Ballal S, Shanbhogue AY, Kamath U, Bhat GS, Bhat KM.

Journal:

J Periodontol (2009): 657

Cu,Zn superoxide dismutase and zinc stress in the metal-tolerant ericoid mycorrhizal fungus Oidiodendron maius Zn

Authors:

Vallino M, Martino E, Boella F, Murat C, Chiapello M, Perotto S.

Journal:

FEMS Microbiol Lett (2009): 48

Enhancement of aglycone, vitamin K2 and superoxide dismutase activity of black soybean through fermentation with Bacillus subtilis BCRC 14715 at different temperatures

Authors:

Wu CH, Chou CC.

Journal:

J Agric Food Chem (2009): 10695

High-level molecular diversity of copper-zinc superoxide dismutase genes among and within species of arbuscular Mycorrhizal fungi

Authors:

Corradi N, Ruffner B, Croll D, Colard A, Horak A, S and ers IR., undefined

Journal:

Appl Environ Microbiol (2009): 1970

Superoxide dismutase and catalase activity in naturally derived commercial surfactants

Authors:

Dani C, Buonocore G, Longini M, Felici C, Rodriguez A, Corsini I, Rubaltelli FF.

Journal:

Pediatr Pulmonol (2009): 1125

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	440 nm
Recommended plate	Clear bottom

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Page updated on October 8, 2026

Component A: WST-1	2 bottles
Component B: SOD Enzyme solution (50X)	1 vial (100 μL)
Component C: NADH	2 vials
Component D: SOD standard	1 vial (500 Units)
Component E: Assay Buffer	1 bottle (20 mL)