Name: Amplite® Colorimetric Total NAD and NADH Assay Kit
Brand: AAT Bioquest
SKU: 15258
Price: 355 USD
Availability: InStock

Amplite® Colorimetric Total NAD and NADH Assay Kit

Amplite® Colorimetric Total NAD and NADH Assay Kit is a colorimetric assay kit for quantifying total NAD and NADH in biological samples.

Detection Principle: Uses an NAD/NADH cycling enzyme reaction for colorimetric detection.
Signal Advantage: Provides more sensitive detection than direct UV absorbance at 340 nm.
Reaction Chemistry: Specifically detects NAD/NADH through an enzyme cycling system.
Instrument Compatibility: Absorbance detection at 570/610 nm using a microplate reader.
Plate Format: Designed for clear-bottom microplates.

NADH dose response was measured with Amplite® Colorimetric Total NAD and NADH Assay Kit in a white/clear bottom 96-well plate using a NOVOStar (BMG Labtech) microplate reader.

Protocol

SDS

Catalog	Size	Price	Quantity
15258	400 Tests	Price

Overview

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+, and NAD+ is the oxidized form of NADH. It forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. NADP is used in anabolic biological reactions, such as fatty acid and nucleic acid synthesis, which require NADPH as a reducing agent. In chloroplasts, NADP is an oxidizing agent important in the preliminary reactions of photosynthesis. The NADPH produced by photosynthesis is then used as reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis. The traditional NAD/NADH and NADP/NADPH assays are done by monitoring of NADH or NADPH absorption at 340 nm. This method suffers low sensitivity and high interference since the assay is done in the UV range that requires expensive quartz microplate. This Amplite® NAD/NADH Assay Kit provides a convenient method for sensitive detection of NAD and NADH. The enzymes in the system specifically recognize NAD/NADH in an enzyme cycling reaction. There is no need to purify NAD/NADH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity.

Citations

View all 64 citations: Citation Explorer

Nicotinamide ribose ameliorates myocardial ischemia/reperfusion injury by regulating autophagy and regulating oxidative stress

Authors:

Yuan, Chen and Yang, Heng and Lan, Wanqi and Yang, Juesheng and Tang, Yanhua

Journal:

Experimental and Therapeutic Medicine (2024): 1--10

NAD+ Synthetase is Required for Free-living and Symbiotic Nitrogen Fixation in the Actinobacterium Frankia casuarinae

Authors:

Kucho, Ken-ichi and Asukai, Koya and Van Nguyen, Thanh

Journal:

Microbes and Environments (2023): ME22093

NAD (H)-loaded nanoparticles for efficient sepsis therapy via modulating immune and vascular homeostasis

Authors:

Ye, Mingzhou and Zhao, Yi and Wang, Yuyuan and Xie, Ruosen and Tong, Yao and Sauer, John-Demian and Gong, Shaoqin

Journal:

Nature Nanotechnology (2022): 1--11

Mycoplasma pneumoniae protects infected epithelial cells from hydrogen peroxide-induced cell detachment

Authors:

Yamamoto, Takeshi and Kida, Yutaka and Kuwano, Koichi

Journal:

Cellular microbiology (2019): e13015

Enhanced 1, 3-propanediol production in Klebsiella pneumoniae by a combined strategy of strengthening the TCA cycle and weakening the glucose effect

Authors:

Lu, Xinyao and Ren, Shunli and Lu, Jingzheng and Zong, Hong and Song, Jian and Zhuge, Bin

Journal:

Journal of applied microbiology (2018)

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	570/610 nm
Recommended plate	Clear bottom

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Page updated on October 8, 2026

Component A: NAD/NADH Recycling Enzyme Mix	2 bottles (lyophilized powder)
Component B: NADH Sensor Buffer	1 bottle (20 mL)
Component C: NADH Standard (FW: 709)	1 vial (142 µg)