Name: Amplite® Fluorimetric Acetylcholine Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 11403
Price: 232 USD
Availability: InStock

Amplite® Fluorimetric Acetylcholine Assay Kit

Red Fluorescence

Acetylcholine and its metabolites are needed for three main physiological purposes: structural integrity and signaling roles for cell membranes, cholinergic neurotransmission (acetylcholine synthesis), and as a major source for methyl groups via its metabolite, trimethylglycine (betaine) that participates in the S-adenosylmethionine synthesis pathways. It plays an important role in the central nervous system as a precursor for acetylcholine and membrane phosphatidylcholine. This Amplite® Fluorimetric Acetylcholine Assay Kit provides one of the most sensitive methods for the quantifying acetylcholine. The kit uses Amplite® Red™ to quantify acetylcholine through choline oxidase-mediated enzyme coupling reactions. The fluorescence intensity of Amplite® Red™ is proportional to acetylcholine.

Acetylcholine dose response was measured in a 96-well solid black plate with Amplite® Fluorimetric Acetylcholine Assay Kit (Cat. # 11403) using a Gemini fluorescence microplate reader (Molecular devices).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11403	200 Tests	Price

Citations

View all 35 citations: Citation Explorer

Branched-chain amino acids govern the high learning ability phenotype in Tokai high avoider (THA) rats

Authors:

Shida, Yukari and Endo, Hitoshi and Owada, Satoshi and Inagaki, Yutaka and Sumiyoshi, Hideaki and Kamiya, Akihide and Eto, Tomoo and Tatemichi, Masayuki

Journal:

Scientific reports (2021): 1--16

Adlay hull extracts attenuate $\beta$-amyloid-induced neurotoxicity and oxidative stress in PC12 cells through antioxidative, anti-inflammatory, and antiapoptotic activities

Authors:

Tsay, Gregory J and Lin, Yu-Ta and Hsu, Chia-Hong and Tang, Feng-Yao and Kuo, Yueh-Hsiung and Chao, Che-Yi

Journal:

Biochemistry and Biophysics Reports (2021): 101020

Fermented rice peptides attenuate scopolamine-induced memory impairment in mice by regulating neurotrophic signaling pathways in the hippocampus

Authors:

Corpuz, Henry M and Fujii, Hiroshi and Nakamura, Soichiro and Katayama, Shigeru

Journal:

Brain Research (2019): 146322

The Additive Effects of Low Dose Intake of Ferulic Acid, Phosphatidylserine and Curcumin, Not Alone, Improve Cognitive Function in APPswe/PS1dE9 Transgenic Mice

Authors:

Okuda, Michiaki and Fujita, Yuki and Sugimoto, Hachiro

Journal:

Biological and Pharmaceutical Bulletin (2019): 1694--1706

Oral Administration of Okara Soybean By-Product Attenuates Cognitive Impairment in a Mouse Model of Accelerated Aging

Authors:

Corpuz, Henry M and Arimura, Misa and Chawalitpong, Supatta and Miyazaki, Keiko and Sawaguchi, Makoto and Nakamura, Soichiro and Katayama, Shigeru

Journal:

Nutrients (2019): 2939

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 16, 2025

Component A: Amplite® Red	1 vial
Component B: Acetylcholine Probe	2 bottles (lyophilized powder)
Component C: Acetylcholine Standard	1 vial
Component D: Assay Buffer	1 bottle (25 mL)
Component E: DMSO	1 vial (100 µL)