Name: Amplite® Fluorimetric Acetylcholine Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 11403
Price: 237 USD
Availability: InStock

Amplite® Fluorimetric Acetylcholine Assay Kit

Red Fluorescence

Amplite® Fluorimetric Acetylcholine Assay Kit *Red Fluorescence* is a fluorimetric assay kit for quantifying acetylcholine with a fluorescence microplate reader.

Detection Principle: Uses choline oxidase-mediated enzyme coupling reactions.
Signal Response: Fluorescence intensity is proportional to acetylcholine concentration.
Instrument Compatibility: Fluorescence detection at Ex 540/Em 590 nm.
Plate Format: Recommended for solid black microplates.

Acetylcholine dose response was measured in a 96-well solid black plate with Amplite® Fluorimetric Acetylcholine Assay Kit (Cat. # 11403) using a Gemini fluorescence microplate reader (Molecular devices).

Protocol

SDS

Catalog	Size	Price	Quantity
11403	200 Tests	Price

Overview

Acetylcholine and its metabolites are needed for three main physiological purposes: structural integrity and signaling roles for cell membranes, cholinergic neurotransmission (acetylcholine synthesis), and as a major source for methyl groups via its metabolite, trimethylglycine (betaine) that participates in the S-adenosylmethionine synthesis pathways. It plays an important role in the central nervous system as a precursor for acetylcholine and membrane phosphatidylcholine. This Amplite® Fluorimetric Acetylcholine Assay Kit provides one of the most sensitive methods for the quantifying acetylcholine. The kit uses Amplite® Red™ to quantify acetylcholine through choline oxidase-mediated enzyme coupling reactions. The fluorescence intensity of Amplite® Red™ is proportional to acetylcholine.

Citations

View all 36 citations: Citation Explorer

M3 muscarinic receptor antagonism attenuates cocaine-induced hippocampal oligodendroglial changes and relapse-like behavior in male mice

Authors:

Yamada, Jun and Maeda, Shoichiro and Okamura, Ayumi and Iinuma, Kyoko M and Jinno, Shozo

Journal:

Neuropharmacology (2026): 111144

Branched-chain amino acids govern the high learning ability phenotype in Tokai high avoider (THA) rats

Authors:

Shida, Yukari and Endo, Hitoshi and Owada, Satoshi and Inagaki, Yutaka and Sumiyoshi, Hideaki and Kamiya, Akihide and Eto, Tomoo and Tatemichi, Masayuki

Journal:

Scientific reports (2021): 1--16

Adlay hull extracts attenuate $\beta$-amyloid-induced neurotoxicity and oxidative stress in PC12 cells through antioxidative, anti-inflammatory, and antiapoptotic activities

Authors:

Tsay, Gregory J and Lin, Yu-Ta and Hsu, Chia-Hong and Tang, Feng-Yao and Kuo, Yueh-Hsiung and Chao, Che-Yi

Journal:

Biochemistry and Biophysics Reports (2021): 101020

Fermented rice peptides attenuate scopolamine-induced memory impairment in mice by regulating neurotrophic signaling pathways in the hippocampus

Authors:

Corpuz, Henry M and Fujii, Hiroshi and Nakamura, Soichiro and Katayama, Shigeru

Journal:

Brain Research (2019): 146322

The Additive Effects of Low Dose Intake of Ferulic Acid, Phosphatidylserine and Curcumin, Not Alone, Improve Cognitive Function in APPswe/PS1dE9 Transgenic Mice

Authors:

Okuda, Michiaki and Fujita, Yuki and Sugimoto, Hachiro

Journal:

Biological and Pharmaceutical Bulletin (2019): 1694--1706

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 6, 2026

Component A: Amplite® Red	1 vial
Component B: Acetylcholine Probe	2 bottles (lyophilized powder)
Component C: Acetylcholine Standard	1 vial
Component D: Assay Buffer	1 bottle (25 mL)
Component E: DMSO	1 vial (100 µL)